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Gene Expression Data
Assay Details
Assay
Reference: J:85723 Heeroma JH, et al., Development of the mouse neuromuscular junction in the absence of regulated secretion. Neuroscience. 2003;120(3):733-44
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5707903
Gene symbol: Syn1
Gene name: synapsin I
Antibody: anti-pan-synapsin (E028)
Detection system: Secondary antibody coupled to fluorescein
Assay notes: The antibody also recognizes other related proteins; all possibilities have been annotated.
Results
Specimen 2A: embryonic day 14.5 (more )
Note: Control; genotype either +/+ or +/-. Also stained for Gap43 (assay MGI:5708140).
Structure Level Pattern Image Note
TS22: phrenic nerve Present 2A
Specimen 2B: embryonic day 14.5; Stxbp1tm1Sud/Stxbp1tm1Sud (more )
Note: Also stained for Gap43 (assay MGI:5708140).
Structure Level Pattern Image Note
TS22: phrenic nerve Present 2B
Specimen 2C: embryonic day 14.5 (more )
Note: Control; genotype either +/+ or +/-. Also stained for Gap43 (assay MGI:5708140).
Structure Level Pattern Image Note
TS22: phrenic nerve Present 2C
Specimen 2D: embryonic day 14.5; Stxbp1tm1Sud/Stxbp1tm1Sud (more )
Note: Also stained for Gap43 (assay MGI:5708140).
Structure Level Pattern Image Note
TS22: phrenic nerve Present 2D
Specimen 2E: embryonic day 14.5 (more )
Note: Control; genotype either +/+ or +/-. Also stained for Gap43 (assay MGI:5708140).
Structure Level Pattern Image Note
TS22: phrenic nerve Present 2E
Specimen 2F: embryonic day 14.5; Stxbp1tm1Sud/Stxbp1tm1Sud (more )
Note: Also stained for Gap43 (assay MGI:5708140).
Structure Level Pattern Image Note
TS22: phrenic nerve Present 2F
Specimen 4A: embryonic day 15.5 (more )
Note: Control; genotype either +/+ or +/-. Double labeled: green - pan-synapsin and Gap43 (assay MGI:5708140); red - alpha-bungarotoxin.
Structure Level Pattern Image Note
TS23: diaphragm Present Regionally restricted 4A Expression is in nerves of the diaphragm.
TS23: peripheral nerve Present Regionally restricted 4A Expression is in nerves of the diaphragm.
TS23: phrenic nerve Present 4A
Specimen 4B: embryonic day 15.5; Stxbp1tm1Sud/Stxbp1tm1Sud (more )
Note: Double labeled: green - pan-synapsin and Gap43 (assay MGI:5708140); red - alpha-bungarotoxin.
Structure Level Pattern Image Note
TS23: diaphragm Present Regionally restricted 4B Expression is in nerves of the diaphragm.
TS23: peripheral nerve Present Regionally restricted 4B Expression is in nerves of the diaphragm.
Specimen 4C: embryonic day 15.5; Stxbp1tm1Sud/Stxbp1tm1Sud (more )
Note: Double labeled: green - pan-synapsin and Gap43 (assay MGI:5708140); red - alpha-bungarotoxin.
Structure Level Pattern Image Note
TS23: diaphragm Present Regionally restricted 4C Expression is in nerves of the diaphragm.
TS23: peripheral nerve Present Regionally restricted 4C Expression is in nerves of the diaphragm.
Specimen 4D: embryonic day 18.5 (more )
Note: Control; genotype either +/+ or +/-. Double labeled: green - pan-synapsin and Gap43 (assay MGI:5708140); red - alpha-bungarotoxin.
Structure Level Pattern Image Note
TS26: diaphragm Present Regionally restricted 4D Expression is in nerves of the diaphragm.
TS26: peripheral nerve Present Regionally restricted 4D Expression is in nerves of the diaphragm.
TS26: phrenic nerve Present 4D
Specimen 4E: embryonic day 18.5; Stxbp1tm1Sud/Stxbp1tm1Sud (more )
Note: Double labeled: green - pan-synapsin and Gap43 (assay MGI:5708140); red - alpha-bungarotoxin.
Structure Level Pattern Image Note
TS26: diaphragm Present 4E Expression is in nerves of the diaphragm.
TS26: peripheral nerve Present 4E Expression is in nerves of the diaphragm.
Specimen 4F: embryonic day 18.5; Stxbp1tm1Sud/Stxbp1tm1Sud (more )
Note: Double labeled: green - pan-synapsin and Gap43 (assay MGI:5708140); red - alpha-bungarotoxin.
Structure Level Pattern Image Note
TS26: diaphragm Present 4F Expression is in nerves of the diaphragm.
TS26: peripheral nerve Present 4F Expression is in nerves of the diaphragm.
Specimen 6A: embryonic day 15.5 (more )
Note: Control; genotype either +/+ or +/-. Double labeled: green - pan-synapsin and Gap43 (assay MGI:5708140); red - alpha-bungarotoxin.
Structure Level Pattern Image Note
TS23: intercostal muscle Present Regionally restricted 6A , 6C Expression is in nerves of the intercostal muscles.
TS23: peripheral nerve Present Regionally restricted 6A , 6C Expression is in nerves of the intercostal muscles.
Specimen 6D: embryonic day 15.5; Stxbp1tm1Sud/Stxbp1tm1Sud (more )
Note: Double labeled: green - pan-synapsin and Gap43 (assay MGI:5708140); red - alpha-bungarotoxin.
Structure Level Pattern Image Note
TS23: intercostal muscle Present Regionally restricted 6D , 6F Expression is in nerves of the intercostal muscles.
TS23: peripheral nerve Present Regionally restricted 6D , 6F Expression is in nerves of the intercostal muscles.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory