About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:147897 Unhavaithaya Y, et al., MILI, a PIWI-interacting RNA-binding protein, is required for germ line stem cell self-renewal and appears to positively regulate translation. J Biol Chem. 2009 Mar 6;284(10):6507-19
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5707857
Gene symbol: Piwil2
Gene name: piwi-like RNA-mediated gene silencing 2
Results
Specimen 3A: postnatal adult (more )
Note: Triple labeled: red - Piwil2; green - laminin; blue - DAPI.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 3A Specific expression in cytoplasm of spermatogonia and pachytene stage spermatocytes.
TS28: spermatocyte Present Regionally restricted 3A No expression in more differentiated germ cells.
TS28: spermatogonium Present Regionally restricted 3A No expression in more differentiated germ cells.
TS28: spermatid Absent 3A
Specimen 3B: postnatal adult (more )
Note: Triple labeled: red - Piwil2; green - laminin; blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 3B
TS28: spermatocyte Present Regionally restricted 3B Specific expression in cytoplasm of spermatogonia and pachytene stage spermatocytes.
TS28: spermatogonium Present Regionally restricted 3B Specific expression in cytoplasm of spermatogonia and pachytene stage spermatocytes.
TS28: spermatid Absent 3B No expression in more differentiated germ cells.
TS28: Sertoli cell Sertoli cell Absent 3B
Specimen 3C: postnatal adult (more )
Note: Triple labeled: red - Piwil2; green - Tsx (assay MGI:5707858); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS28: seminiferous tubule epithelium Present 3C
TS28: spermatocyte Present 3C
TS28: spermatogonium Present 3C
TS28: Sertoli cell Sertoli cell Absent 3C
Specimen 3D: postnatal adult (more )
Note: Triple labeled: red - Piwil2; green - Ge-1 (Edc4; assay MGI:5707859); blue - DAPI.
Structure Level Pattern Image Note
TS28: germ cell of testis Present Regionally restricted 3D In the germ line cytoplasm, expression enriched in spherical structures.
Specimen 3F/3H: postnatal adult (more )
Note: Triple labeled: red - Piwil2; green - Plzf (Zbtb16; assay MGI:5707860); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatogonium Present 3H , 3F Expression in all spermatogonia.
Specimen S2C: postnatal adult; Piwil2tm1Nkn/Piwil2+ (more )
Note: Triple labeled: red - Piwil2; green - Tsx (assay MGI:5707858); blue - DAPI.
Structure Level Pattern Image Note
TS28: germ cell of testis Present S2C
Specimen S2D: postnatal adult; Piwil2tm1Nkn/Piwil2tm1Nkn (more )
Note: Triple labeled: red - Piwil2; green - Tsx (assay MGI:5707858); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Absent S2D
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory