Gene Expression Data
Assay Details
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Assay
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Reference:
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J:139734
Hafler BP, et al., Expression and function of Nkx6.3 in vertebrate hindbrain. Brain Res. 2008 Jul 30;1222:42-50
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5707643
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Gene symbol:
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Nkx6-3
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Gene name:
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NK6 homeobox 3
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Probe:
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Nkx6-3 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 10.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1C:
embryonic day 10.5
(more )
Note: D is a confocal higher magnification image. Double labeled: red (pseudocolored) - Nkx6-3; green - Lhx3 (assay MGI:5707656).
Specimen
1C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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D is a confocal higher magnification image. Double labeled: red (pseudocolored) - Nkx6-3; green - Lhx3 (assay MGI:5707656).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere 7 |
Present |
Regionally restricted |
1C
,
1D
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Expression is in V2 neurons.
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Specimen
1E:
embryonic day 12.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain |
Present |
Regionally restricted |
1E
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Expression is in the V2a interneuron and motor neuron populations in the caudal hindbrain.
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Specimen
1G:
embryonic day 12.5
(more )
Note: H is a confocal higher magnification image. Double labeled: red (pseudocolored) - Nkx6-3; green - Vsx2 (assay MGI:5707659).
Specimen
1G:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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H is a confocal higher magnification image. Double labeled: red (pseudocolored) - Nkx6-3; green - Vsx2 (assay MGI:5707659).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain |
Present |
Regionally restricted |
1G
,
1H
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Expression is in the V2a interneuron and motor neuron populations in the caudal hindbrain.
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Specimen
1I:
embryonic day 12.5
(more )
Note: J is a confocal higher magnification image. Double labeled: red (pseudocolored) - Nkx6-3; green - Gata3 (assay MGI:5707666).
Specimen
1I:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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J is a confocal higher magnification image. Double labeled: red (pseudocolored) - Nkx6-3; green - Gata3 (assay MGI:5707666).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain |
Present |
Regionally restricted |
1I
,
1J
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Expression is in the V2a interneuron and motor neuron populations in the caudal hindbrain.
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Specimen
1K:
embryonic day 12.5
(more )
Note: L is a confocal higher magnification image. Double labeled: red (pseudocolored) - Nkx6-3; green - Lhx3 (assay MGI:5707656).
Specimen
1K:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
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L is a confocal higher magnification image. Double labeled: red (pseudocolored) - Nkx6-3; green - Lhx3 (assay MGI:5707656).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain |
Present |
Regionally restricted |
1K
,
1L
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Expression is in the V2a/b interneuron and motor neuron populations in the caudal hindbrain.
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Specimen
2A:
embryonic day 12.5
(more )
Note: D is a confocal higher magnification image. Double labeled: red (pseudocolored) - Nkx6-3; green - Mnx1 (assay MGI:5707668).
Specimen
2A:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
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D is a confocal higher magnification image. Double labeled: red (pseudocolored) - Nkx6-3; green - Mnx1 (assay MGI:5707668).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain |
Present |
Regionally restricted |
2D
,
2A
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Expression is in postmitotic V2 interneurons and is absent in motor neurons in the caudal hindbrain.
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Specimen
2B:
embryonic day 12.5
(more )
Note: E is a confocal higher magnification image. Double labeled: red (pseudocolored) - Nkx6-3; green - Nkx6-1 (assay MGI:5707671).
Specimen
2B:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
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E is a confocal higher magnification image. Double labeled: red (pseudocolored) - Nkx6-3; green - Nkx6-1 (assay MGI:5707671).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain |
Present |
Regionally restricted |
2E
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2B
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Expression is in postmitotic V2 interneurons in the caudal hindbrain.
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Specimen
2C:
embryonic day 12.5
(more )
Note: F is a confocal higher magnification image. Double labeled: red (pseudocolored) - Nkx6-3; green - Mki67 (assay MGI:5707675).
Specimen
2C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
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F is a confocal higher magnification image. Double labeled: red (pseudocolored) - Nkx6-3; green - Mki67 (assay MGI:5707675).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain |
Present |
Regionally restricted |
2C
,
2F
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Expression is in postmitotic V2 interneurons in the caudal hindbrain.
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Specimen
2G:
embryonic day 12.5
(more )
Specimen
2G:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain |
Present |
Regionally restricted |
2G
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Expression is in V2a interneurons in the caudal hindbrain.
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Specimen
2H:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2tm1Rth
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain |
Present |
Regionally restricted |
2H
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The expression domain is expanded in this mutant.
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Specimen
S1 A:
embryonic day 12.5
(more )
Specimen
S1 A:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain |
Present |
Regionally restricted |
S1A
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Expression is in the caudal hindbrain.
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Specimen
S1 B:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Nkx6-3tm1Shiv/Nkx6-3tm1Shiv
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools