Pcsk2 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:35634 Marcinkiewicz M, et al., Ontogeny of the prohormone convertases PC1 and PC2 in the mouse hypophysis and their colocalization with corticotropin and alpha-melanotropin. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):4922-6
Assay type:	Northern blot
MGI Accession ID:	MGI:5707068
Gene symbol:	Pcsk2
Gene name:	proprotein convertase subtilisin/kexin type 2

Probe:	mPC2 probe1
Probe preparation:	Antisense labelled with P32 RNA
Visualized with:	Autoradiography

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Amount	Genetic Background	Mutant Allele(s)	Sex
P1	P1	TS27: adenohypophysis pars intermedia	Present	10 µg; total RNA	CD-1		Not Specified
P7	P7	TS28: adenohypophysis pars intermedia	Present	10 µg; total RNA	CD-1		Not Specified
P14	P14	TS28: adenohypophysis pars intermedia	Present	10 µg; total RNA	CD-1		Not Specified
P28	P28	TS28: adenohypophysis pars intermedia	Strong (b)	10 µg; total RNA	CD-1		Not Specified
P42	P42	TS28: adenohypophysis pars intermedia	Strong	10 µg; total RNA	CD-1		Not Specified
P56	P56	TS28: adenohypophysis pars intermedia	Strong (c)	10 µg; total RNA	CD-1		Not Specified

Notes:
(a) Expression increased gradually from the first postnatal day to adulthood.
(b) Authors report maximal levels of expression at P28, with no appreciable decline until adulthood.
(c) No appreciable decline in expression until adulthood.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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