Antibody:	anti-PD-1 (clone 29F.1A12)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: neural retinal epithelium	Absent		3A upper left

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Weak	Regionally restricted	3A upper right	Expressed in the inner and outer neuroblast layers.

Structure	Level	Pattern	Image	Note
TS25: retina nuclear layer	Weak	Regionally restricted	3A middle left	Expressed throughout the ganglion cell layer, which contained retinal ganglion cells; and the neuroblast layer, which contained migrating precursors and retinal progenitors.

Structure	Level	Pattern	Image	Note
TS27: retina layer	Present	Regionally restricted	3A middle right	Greatest expression. Relatively high levels expression in the inner retina. Substantial expression was also detected in a subset of proliferating neuroblasts occupying the ventricular zone.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present		3A lower left	Greatest expression.
TS28: retina inner nuclear layer	Present		3A lower left	Greatest expression.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present		3A lower right	Decreased expression.
TS28: retina inner nuclear layer	Present		3A lower right	Decreased expression.