Gene Expression Data
Assay Details
Assay
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Reference:
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J:98542
Rowan S, et al., A POU factor binding site upstream of the Chx10 homeobox gene is required for Chx10 expression in subsets of retinal progenitor cells and bipolar cells. Dev Biol. 2005 May 15;281(2):240-55
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5706741
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Gene symbol:
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Nes
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Gene name:
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nestin
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Probe:
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UI-M-CG0p-bdb-e-07-0-UI.s1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5A E13.5:
embryonic day 13.5
(more )
Specimen
5A E13.5:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
5A E16.5:
embryonic day 16.5
(more )
Specimen
5A E16.5:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
5A P0:
postnatal day 0
(more )
Specimen
5A P0:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Structure |
Level |
Pattern |
Image |
Note |
TS27: neural retina |
Weak |
Regionally restricted |
5A P0 Nestin
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Weak expression was detected in the outer neuroblastic layer.
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Specimen
not shown:
embryonic day 14.5
(more )
Specimen
not shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
5C E14.5 orJ/+:
embryonic day 14.5; Vsx2or-J/Vsx2 +
(more )
Specimen
5C E14.5 or/+:
(close )
Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Vsx2or-J/Vsx2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
5C E14.5 or/or:
(close )
Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Vsx2or-J/Vsx2or-J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
5C E17.5 orJ/+:
embryonic day 17.5; Vsx2or-J/Vsx2 +
(more )
Specimen
5C E17.5 or/+:
(close )
Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Vsx2or-J/Vsx2+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
5C E17.5 or/or:
(close )
Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Vsx2or-J/Vsx2or-J
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Structure |
Level |
Pattern |
Image |
Note |
TS25: retina |
Present |
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5C E17.5 orJ/orJ
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There was no difference in expression level between heterozygous and homozygous animals.
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