Pou3f2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:98542 Rowan S, et al., A POU factor binding site upstream of the Chx10 homeobox gene is required for Chx10 expression in subsets of retinal progenitor cells and bipolar cells. Dev Biol. 2005 May 15;281(2):240-55
Assay type:	RNA in situ
MGI Accession ID:	MGI:5706730
Gene symbol:	Pou3f2
Gene name:	POU domain, class 3, transcription factor 2

Probe:	Brn2 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 5A E13.5: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: neural retinal epithelium	Trace	Regionally restricted	5A E13.5 Brn-2	Expression was barely detectable and was concentrated in the inner neuroblastic layer and weakly in the outer neuroblastic layer.

Specimen 5A E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	5A E16.5 Brn-2	Expression was relatively higher in the outer neuroblastic layer and lower in the inner neuroblastic layer.

5A P0 Brn-2 ©

Specimen 5A P0: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: neural retina	Present	Regionally restricted	5A P0 Brn-2	Expression was detected in the outer neuroblastic layer.
TS27: retina ganglion cell layer	Present		5A P0 Brn-2

Specimen 5B E12.5: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: retina	Trace	Regionally restricted	5B E12.5 Brn-2	Very low level expression was detected only in the center of the retina.

Specimen 5B E17.5: embryonic day 17.5 (more

)

Structure	Level	Pattern	Image	Note
TS25: neural retina	Weak	Regionally restricted	5B E17.5 Brn-2	Expression was lower than at E16.5. Weak expression was in the inner part of the inner neuroblastic layer.

Specimen not shown E14.5: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: retina	Present

Specimen not shown E14.5 or^J/or^J: embryonic day 14.5; Vsx2^or-J/Vsx2^or-J (more

)

Structure	Level	Pattern	Image	Note
TS22: retina	Present			There was no change in expression level in the mutant.

Specimen not shown E17.5: embryonic day 17.5 (more

)

Structure	Level	Pattern	Image	Note
TS25: retina	Present

Specimen not shown E17.5 or^J/or^J: embryonic day 17.5; Vsx2^or-J/Vsx2^or-J (more

)

Structure	Level	Pattern	Image	Note
TS25: retina	Present			There was no change in expression level in the mutant.

7A ©

Specimen 7A: postnatal day 6 (more

)

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Non-Uniform	7A	Expression was in some, but not all, bipolar and amacrine cells.
TS28: retina ganglion cell layer	Present		7A

7B ©

Specimen 7B: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Non-Uniform	7B	Expression was in some, but not all, bipolar and amacrine cells.
TS28: retina ganglion cell layer	Present		7B

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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