H2ax Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:186180 Reijns MA, et al., Enzymatic removal of ribonucleotides from DNA is essential for mammalian genome integrity and development. Cell. 2012 May 25;149(5):1008-22
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5705109
Gene symbol:	H2ax
Gene name:	H2A.X variant histone

Antibody:	anti-Phospho-Histone H2A.X (Ser139)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

Specimen 4A control: embryonic day 6.5 (more

)
Note: Double labeled: red - H2ax; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS8: epiblast	Ambiguous		4A control

Specimen 4A mutant: embryonic day 6.5; Rnaseh2b^{tm1a(EUCOMM)Wtsi}/Rnaseh2b^{tm1a(EUCOMM)Wtsi} (more

)
Note: Double labeled: red - H2ax; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS8: epiblast	Present	Regionally restricted	4A mutant	No difference in levels of expression, but elevated nuclear expression in mutants compared with wild type embryos.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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