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Gene Expression Data
Assay Details
Assay
Reference: J:186180 Reijns MA, et al., Enzymatic removal of ribonucleotides from DNA is essential for mammalian genome integrity and development. Cell. 2012 May 25;149(5):1008-22
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5705107
Gene symbol: Rnaseh2a
Gene name: ribonuclease H2, large subunit
Antibody: RNase H2 complex antibody (cone 62)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3B: embryonic day 7.5 (more )
Note: Triple labeled: green - Rnaseh2a, Rnaseh2b, and Rnaseh2c; red Ki67 (Mki67; assay MGI:5705108); blue DAPI.
Structure Level Pattern Image Note
TS11: embryo ectoderm Present Ubiquitous 3B Expression in all three cell layers of gastrulating mouse embryos.
TS11: embryo endoderm Present Ubiquitous 3B Expression in all three cell layers of gastrulating mouse embryos.
TS11: embryo mesoderm Present Ubiquitous 3B Expression in all three cell layers of gastrulating mouse embryos.
Specimen S2B RNase H2 null: embryonic day 7.5; Rnaseh2btm1a(EUCOMM)Wtsi/Rnaseh2btm1a(EUCOMM)Wtsi (more )
Note: Triple labeled: green - Rnaseh2a, Rnaseh2b, and Rnaseh2c; red Ki67 (Mki67; assay MGI:5705108); blue DAPI.
Structure Level Pattern Image Note
TS11: embryo Absent S2B RNaseH2 null
Specimen S2C: embryonic day 8.5 (more )
Note: Double labeled: green RNase H2; blue DAPI.
Structure Level Pattern Image Note
TS13: embryo Present Ubiquitous S2C
Specimen S2D: embryonic day 13.5 (more )
Note: Double labeled: green RNase H2; blue DAPI.
Structure Level Pattern Image Note
TS21: embryo Present Regionally restricted S2D Expression restricted to highly proliferative tissues.
TS21: dermis Strong Widespread S2D
TS21: epidermis Strong Widespread S2D
TS21: cartilage condensation Weak S2D Greatly reduced expression in underlying skeletal muscle and cartilaginous structures.
TS21: skeletal muscle Weak S2D Greatly reduced expression in underlying skeletal muscle and cartilaginous structures.
Specimen S2E: embryonic day 13.5 (more )
Note: Triple labeled: green - Rnaseh2a, Rnaseh2b, and Rnaseh2c; red Ki67 (Mki67; assay MGI:5705108); blue DAPI.
Structure Level Pattern Image Note
TS21: cerebral cortex subventricular zone Strong Regionally restricted S2E Expression restricted to proliferating progenitor cells and recently generated neurons.
TS21: telencephalon ventricular layer Strong Regionally restricted S2E Expression restricted to proliferating progenitor cells and recently generated neurons.
Specimen S2F: postnatal day 6 (more )
Note: Double labeled: green RNase H2; blue DAPI.
Structure Level Pattern Image Note
TS28: hair follicle Strong Regionally restricted S2F Expression restricted to proliferating epidermal progenitors in anagen hair follicles.
Specimen not shown: postnatal day 6 (more )
Structure Level Pattern Image Note
TS28: epidermis basal layer Present Regionally restricted Expression in basal layer of interfollicular epidermis
Specimen S2G: postnatal day 150 (more )
Note: Triple labeled: green - Rnaseh2a, Rnaseh2b, and Rnaseh2c; red Ki67 (Mki67; assay MGI:5705108); blue DAPI.
Structure Level Pattern Image Note
TS28: small intestine crypt of lieberkuhn Strong S2G Expression in crypts of Lieberkuhn, between villi of mucosa.
Specimen S2H: postnatal day 150 (more )
Note: Triple labeled: green - Rnaseh2a, Rnaseh2b, and Rnaseh2c; red Ki67 (Mki67; assay MGI:5705108); blue DAPI.
Structure Level Pattern Image Note
TS28: testis Present Regionally restricted S2H Expression in outer edges of seminiferous tubules.
TS28: germ cell of testis Present Regionally restricted S2H Expression in spermatogonia at base of epithelium. Low levels of expression in spermatocytes. No expression in spematids or spermatozoa.
Specimen not shown: postnatal (more )
Structure Level Pattern Image Note
TS28: esophagus basal epithelium Present
TS28: spleen germinal center Present
TS28: spleen red pulp Present
TS28: thymus cortex Present
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory