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Tg(Cck-EGFP/RNAi:Gad1)2Mirn
Transgene Detail
Summary
Symbol: Tg(Cck-EGFP/RNAi:Gad1)2Mirn
Name: transgene insertion 2, Karoly Mirnics
MGI ID: MGI:5703888
Synonyms: CCKGAD1TG, Tg(Cck-eGFP/miRNA:GAD1)2KM
Transgene: Tg(Cck-EGFP/RNAi:Gad1)2Mirn  Location: unknown  
Alliance: Tg(Cck-EGFP/RNAi:Gad1)2Mirn page
Transgene
origin
Strain of Origin:  C57BL/6NHsd
Transgene
description
Transgene Type:    Transgenic (Conditional ready, Knockdown, Reporter)
Mutation:    Insertion
  Tg(Cck-EGFP/RNAi:Gad1)2Mirn involves 1 genes/genome features (Gad1) View all
 
Mutation detailsThe transgenic construct uses 116 kbp from the bacterial artificial chromosome (BAC) RP23-60I1 containing the cholecystokinin locus (Cck) as well as ~55 kbp of centromeric sequences (including a portion of the Trak1 locus) and ~56 kbp of telomeric sequences; no other confirmed complete genes are present in the flanking sequences. Using homologous recombination/BAC recombineering, the following was inserted in-frame with the start codon of the BAC-encoded Cck: eGFP, a lox2272 site, a human -globin minigene (exon1-intron-exon2) with the intron containing a miRNA:Gad1 sequence, a second lox2272 site and an SV40 polyA signal. An FRT-flanked neomycin resistance cassette was also present, but removed during BAC recombineering in vitro. The miRNA:Gad1 sequence is a fused miR30,miRNA:Gad1 synthetic miRNA sequence directed against the mouse glutamic acid decarboxylase 1 (Gad1 or GAD67) mRNA (mp ID 283874), with unique flanking miR-30 sequences. No other loci on the BAC were altered. (J:227900)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:227900 Schmidt MJ, et al., Modulation of behavioral networks by selective interneuronal inactivation. Mol Psychiatry. 2014 May;19(5):580-7
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory