Probe:	pT7T3.fz10(1.0)
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Weak	Regionally restricted	1a	Expression is in the anterior half.

Structure	Level	Pattern	Image	Note
TS13: embryo	Strong	Regionally restricted	1b	Expression is in the entire embryo except heart anlage, primitive streak, and extraembryonic tissues.
TS13: neural ectoderm	Present	Widespread
TS13: primitive streak	Weak		1b
TS13: extraembryonic component	Absent		1b
TS13: heart	Absent		1b

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Strong
TS15: future midbrain	Strong	Regionally restricted	1c	Expression is in the dorsal anterior midbrain.
TS15: future spinal cord	Present		1c
TS15: mesonephros mesenchyme	Present		1c
TS15: optic vesicle	Present		1c

Structure	Level	Pattern	Image	Note
TS17: eye	Present		1d
TS17: fronto-nasal process	Present		1d
TS17: future spinal cord	Present		1d
TS17: hindbrain	Present		1d
TS17: mesonephric tubule	Present		1d
TS17: midbrain	Present	Regionally restricted	1d	Expression is in two horizontal stripes.
TS17: forelimb bud	Present	Regionally restricted	1d	Expression is in the handplate.
TS17: 1st branchial arch	Weak		1d
TS17: 2nd branchial arch	Weak		1d
TS17: somite	Weak	Regionally restricted	1d	Expression is in the posterior half.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Strong		1e
TS19: hindlimb bud	Strong		1e
TS19: jaw primordium	Strong		1e

Structure	Level	Pattern	Image	Note
TS20: brain	Strong		1f
TS20: face	Strong		1f
TS20: spinal cord	Strong		1f
TS20: eye	Strong	Regionally restricted	1f	Expression is in the optic cup.
TS20: forelimb mesenchyme	Strong	Regionally restricted	1f	Expression is surrounding the precartilagenous condensations.
TS20: hindlimb mesenchyme	Strong	Regionally restricted	1f	Expression is surrounding the precartilagenous condensations.

Structure	Level	Pattern	Image	Note
TS15: 2nd branchial arch	Present	Regionally restricted	2_left panel	Expression is in spots close to the branchial clefts.
TS15: 3rd branchial arch	Present	Regionally restricted	2_left panel	Expression is in spots close to the branchial clefts.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Strong	Regionally restricted	2a	Expression is in the dorsal part.
TS15: future midbrain	Strong	Regionally restricted	2a	Expression is in the dorsal part.

Structure	Level	Pattern	Image	Note
TS15: optic cup	Present	Regionally restricted	2b	Expression is in the lateral surface.
TS15: future hindbrain	Present	Regionally restricted	2b	Expression is in the dorsal part.

Structure	Level	Pattern	Image	Note
TS15: mesonephros mesenchyme	Present		2c
TS15: future spinal cord	Present	Regionally restricted	2c	Expression is in a medial-ventral stripe at the anterior end of the spinal cord.
TS15: future hindbrain	Present	Regionally restricted	2c	Expression is in a medial-ventral stripe.

Structure	Level	Pattern	Image	Note
TS15: neural plate	Present	Regionally restricted	2d	Expression is in the entire neural plate before neural tube closure at the posterior end of the embryo.

Structure	Level	Pattern	Image	Note
TS20: arch of aorta	Strong		3a
TS20: mandible primordium	Strong		3a
TS20: outflow tract aortic component	Strong		3a
TS20: pericardium	Strong		3a
TS20: vertebral cartilage condensation	Strong		3a
TS20: dorsal aorta	Strong		3a	Including the aortic valve.
TS20: genital tubercle	Strong	Regionally restricted	3a	Expression is at the base.
TS20: atrioventricular endocardial cushion	Strong	Regionally restricted	3a	Expression is in the right atrio-ventricular bulbar cushion tissue.
TS20: tongue	Strong	Regionally restricted	3a	Expression is in the base of tongue.
TS20: chondrocranium	Strong	Regionally restricted	3a	Expression is in the condensations of the basioccipital and basisphenoid bones.
TS20: spinal cord	Strong	Regionally restricted	3a	Expression is in ependymal cells.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Strong		3b
TS20: atrioventricular endocardial cushion	Present		3b
TS20: mandible primordium	Present		3b
TS20: outflow tract aortic component	Present		3b
TS20: pericardium	Present		3b
TS20: vertebral cartilage condensation	Present		3b
TS20: tongue	Present	Regionally restricted	3b	Expression is in the base of tongue.
TS20: genital tubercle	Present	Regionally restricted	3b	Expression is at the base.
TS20: chondrocranium	Present	Regionally restricted	3b	Expression is in the condensations of the basioccipital and basisphenoid bones.
TS20: hindbrain	Present	Regionally restricted	3b	Expression is in ependymal cells.
TS20: spinal cord	Present	Regionally restricted	3b	Expression is in ependymal cells.

Structure	Level	Pattern	Image	Note
TS20: lens vesicle anterior epithelium	Strong		3c
TS20: retina	Strong		3c
TS20: jaw primordium mesenchyme	Strong	Regionally restricted	3c	Expression is in the upper jaw.
TS20: digit mesenchyme	Strong	Regionally restricted	3c	Expression is in mesenchymal cells surrounding the phalangeal condensations.

Structure	Level	Pattern	Image	Note
TS20: gut mesentery	Present		3d
TS20: metanephros	Present		3d
TS20: outflow tract	Present		3d
TS20: rib pre-cartilage condensation	Present		3d
TS20: jaw primordium mesenchyme	Present	Regionally restricted	3d	Expression is in mesenchymal condensations of the upper and lower jaw region.
TS20: forelimb digit	Present	Regionally restricted	3d	Expression is in the joints between the phalanges.
TS20: spinal cord	Present	Regionally restricted	3d	Expression is in the ependymal layer of the medial-ventral aspect of the spinal cord.
TS20: medulla oblongata	Present	Regionally restricted	3d	Expression is in the ependymal layer of the medial-ventral aspect.

Structure	Level	Pattern	Image	Note
TS22: thalamus	Strong		3e
TS22: lower jaw	Present		3e
TS22: metanephros	Present		3e
TS22: nasal mesenchyme	Present		3e
TS22: palatal shelf mesenchyme	Present		3e
TS22: tongue	Present		3e
TS22: vertebral cartilage condensation	Present		3e
TS22: central nervous system	Present	Regionally restricted	3e	Expression is in ependymal cells of various ventricles.
TS22: genital tubercle	Present	Regionally restricted	3e	Expression is at the base.

Structure	Level	Pattern	Image	Note
TS22: lens anterior epithelium	Strong		3f
TS22: retina	Strong		3f
TS22: upper jaw	Strong		3f
TS22: 3rd ventricle	Strong	Regionally restricted	3f	Expression is in ependymal cells.
TS22: hindbrain	Strong	Regionally restricted	3f	Expression is in ependymal cells.
TS22: lateral ventricle	Strong	Regionally restricted	3f	Expression is in ependymal cells.
TS22: spinal cord	Strong	Regionally restricted	3f	Expression is in ependymal cells.

Structure	Level	Pattern	Image	Note
TS26: renal cortex	Present	Regionally restricted	4a , 4b	Expression is in the aggregating mesenchyme and epithelial bodies of the cortical induction and maturation zone.
TS26: comma-shaped body	Present		4a , 4b
TS26: s-shaped body	Weak		4b , 4a

Structure	Level	Pattern	Image	Note
TS20: metanephros	Present	Regionally restricted	5_Wnt-4/+	Expression is in the epithelial bodies of the metanephric mesenchyme.
TS20: mesenchyme of metanephros	Present	Regionally restricted	5_Wnt-4/+	Expression is in the mesenchyme surrounding the ureter.

Structure	Level	Pattern	Image	Note
TS20: mesenchyme of metanephros	Present	Regionally restricted	5_Wnt-4/Wnt-4	Expression is in the mesenchyme surrounding the uereter.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted		Expression is in the ependymal cell layer.
TS17: hindbrain	Present	Regionally restricted		Expression is in the ependymal cell layer.
TS17: midbrain	Present	Regionally restricted		Expression is in the ependymal cell layer.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted		Expression is in the ependymal cell layer.
TS19: hindbrain	Present	Regionally restricted		Expression is in the ependymal cell layer.
TS19: midbrain	Present	Regionally restricted		Expression is in the ependymal cell layer.

Structure	Level	Pattern	Image	Note
TS24: descending aorta	Present
TS24: spinal cord	Present	Regionally restricted		Expression is in the ependymal cell layer.

Structure	Level	Pattern	Image	Note
TS26: metanephros	Present	Spotted