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Gene Expression Data
Assay Details
Assay
Reference: J:226614 Costello I, et al., Lhx1 functions together with Otx2, Foxa2, and Ldb1 to govern anterior mesendoderm, node, and midline development. Genes Dev. 2015 Oct 15;29(20):2108-22
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5701410
Gene symbol: T
Gene name: brachyury, T-box transcription factor T
Antibody: anti-Brachury (N-19; sc-17743)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: The authors described two antibodies but did not detail which was used to obtain these results; it is possible MGI:5439006 was used instead.
Results
Specimen 3B Control: embryonic day 7.5 (more )
Note: early headfold stage
Structure Level Pattern Image Note
TS11: node Present 3B Control
TS11: primitive streak Present 3B Control
TS11: embryo Present Regionally restricted 3B Control Expression is seen at the anterior midline.
Specimen 3B Mutant: embryonic day 7.5; Lhx1tm1Bhr/Lhx1tm2.1Rob, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant. early headfold stage
Structure Level Pattern Image Note
TS11: embryo Present Regionally restricted 3B Lhx1 delta Epi Unlike in wild type, the expression seen at the anterior midline is patchy.
Specimen 3C Control: embryonic day 8.0 (more )
Note: 4-5 somite stage
Structure Level Pattern Image Note
TS13: embryo Present Regionally restricted 3C Control Expression is seen at the anterior midline.
Specimen 3C Mutant: embryonic day 8.0; Lhx1tm1Bhr/Lhx1tm2.1Rob, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant. 4-5 somite stage
Structure Level Pattern Image Note
TS13: embryo Present Regionally restricted 3C Lhx1 delta Epi Unlike in wild type, the expression seen at the anterior midline is patchy.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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