Summary | Mutation origin | Mutation description | Recombinase activity | Phenotypes | Find Mice (IMSR) | References

Symbol:	Nkx2-1tm2.1(flpo)Zjh
Name:	NK2 homeobox 1; targeted mutation 2.1, Z Josh Huang
MGI ID:	MGI:5700385
Synonyms:	Nkx2.1-Flp, Nkx2.1-ires-Flpo
Gene:	Nkx2-1  Location: Chr12:56578741-56583570 bp, - strand  Genetic Position: Chr12, 24.42 cM, cytoband C1-C3
Alliance:	Nkx2-1tm2.1(flpo)Zjh page

Germline Transmission:	Earliest citation of germline transmission: J:235123
Parent Cell Line:	v6.5 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Allele Type:    Targeted (Recombinase) Mutation:    Insertion   Nkx2-1tm2.1(flpo)Zjh expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Nkx2-1 (MGI:108067)   Mutation details: The targeting vector is designed to insert an internal ribosome entry site (IRES) fused to flpo recombinase gene (a codon-optimized FLPe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice), followed by a loxP-flanked neo cassette, downstream of the stop codon of the gene. Cre-mediated recombination removed the floxed neo cassette. (J:235123)

Activity:	Tissue activity of this recombinase allele
Driver:	Nkx2-1 (mouse) Summary of all recombinase alleles driven by Nkx2-1. Alliance mouse Nkx2-1 gene page

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Nkx2-1 Mutation:	24 strains or lines available

Original:	J:235123 He M, et al., Strategies and Tools for Combinatorial Targeting of GABAergic Neurons in Mouse Cerebral Cortex. Neuron. 2016 Sep 21;91(6):1228-1243
All:	10 reference(s)