Probe:	Ndn probe4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS16: central nervous system	Present		1A	Expression in the neural tube.
TS16: 1st branchial arch mandibular component	Present		1A
TS16: forelimb bud	Present		1A
TS16: hindlimb bud	Present		1A
TS16: somite	Present		1A

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Widespread	1B	Wide expression mostly in ventral part of neural tube.

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Present	Regionally restricted	1C	Expression along ventral part of neural tube.
TS21: telencephalon	Present	Regionally restricted	1C	Expression in dorsal telencephalon and septal area.
TS21: diencephalon	Present		1C

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		1D WT

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Absent		1D KO

Structure	Level	Pattern	Image	Note
TS16: central nervous system	Present	Regionally restricted	1E	Expression in the neural tube, particularly in the forebrain and midbrain.
TS16: future forebrain	Present		1E
TS16: future midbrain	Present		1E
TS16: somite	Present		1E

Structure	Level	Pattern	Image	Note
TS16: future brain	Present	Regionally restricted	1F	Expression in the most external cell layer of the neural tube (the earliest born neurons).
TS16: diencephalon	Present	Regionally restricted	1F	Expression in hypothalamic primordium in newly formed neurons at pial surface.
TS16: 1st branchial arch	Present

Structure	Level	Pattern	Image	Note
TS16: future brain marginal layer	Present		1G

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Present		1H
TS20: trigeminal ganglion	Present		1H
TS20: vagus ganglion	Present		1H
TS20: vestibulo-cochlear ganglion	Present		1H
TS20: cranial ganglion	Present	Regionally restricted	1H	Expression in cranial accessory ganglia.
TS20: skeletal muscle	Present	Regionally restricted	1H	Expression in axial muscles.

Structure	Level	Pattern	Image	Note
TS20: nasal septum	Present	Regionally restricted	1I	Expression in a cord of migrating neurons.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present	Regionally restricted	1I'	Expression in sensory nasal (olfactory) epithelium in neurons migrating to the hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	1J left	Expression domain lining the third ventricle.
TS20: prethalamus	Present		1J left	Expression in the ventral thalamus.
TS20: thalamus	Present	Regionally restricted	1J left	Expression in the zona limitans intrathalamica.
TS20: hypothalamus	Present	Regionally restricted	1J left	Expression in the anterior hypothalamus.
TS20: neurohypophysis	Present	Regionally restricted	1J left	Expression in the anterior hypothalamus.

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present	Regionally restricted		Expression in the preplate.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	1K	Expression in telencephalic neuroepithelium.
TS21: diencephalon	Present		1K
TS21: midbrain tegmentum	Present		1K
TS21: cerebellum marginal layer	Present		1K
TS21: medulla oblongata	Present		1K

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	1L	Expression in telencephalic neuroepithelium.
TS21: diencephalon	Present		1L

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		1M
TS21: ventral grey horn	Present	Regionally restricted	1M	Expression in developing post-mitotic neuron precursors.
TS21: dorsal grey horn	Weak	Regionally restricted	1M	Low level of expression in small population on dorsal neurons.
TS21: alar columns	Present	Scattered	1M	Expression in dispersed cells of alar plate.
TS21: spinal cord ventricular layer	Absent		1M
TS21: spinal cord white matter	Absent		1M

Structure	Level	Pattern	Image	Note
TS21: sympathetic ganglion	Present

Structure	Level	Pattern	Image	Note
TS27: cortical subplate	Present		1O
TS27: cerebral cortex marginal zone	Weak		1O

Structure	Level	Pattern	Image	Note
TS16: diencephalon	Present	Regionally restricted	2A	Expression in presumptive hypothalamus in newly formed neurons at pial surface of third ventricle.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	2C	Expression in dorsal hypothalamus.

Structure	Level	Pattern	Image	Note
TS21: cortical preplate	Present	Regionally restricted	2E	Expression in postmitotic neuros of preplate near pial surface.
TS21: cerebral cortex intermediate zone	Absent		2E
TS21: cerebral cortex ventricular layer	Absent		2E

Structure	Level	Pattern	Image	Note
TS20: trigeminal ganglion	Present		3A

Structure	Level	Pattern	Image	Note
TS20: pons	Present	Regionally restricted	3C	Expression in the medial raphe nucleus.

Structure	Level	Pattern	Image	Note
TS20: vomeronasal organ	Present		3E