Gene Expression Data
Assay Details
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Assay
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Reference:
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J:79931
Ohkubo Y, et al., Coordinate regulation and synergistic actions of BMP4, SHH and FGF8 in the rostral prosencephalon regulate morphogenesis of the telencephalic and optic vesicles. Neuroscience. 2002;111(1):1-17
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5700066
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Gene symbol:
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Fgf8
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Gene name:
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fibroblast growth factor 8
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Probe:
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Fgf8 cDNA13
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5A:
embryonic day 8.5; Shhtm1Chg/Shh +
(more  )
Note: 10 somite stage
Specimen
5A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Shhtm1Chg/Shh+
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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10 somite stage
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Specimen
5B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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10 somite state
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Specimen
5C:
embryonic day 9.0; Shhtm1Chg/Shh +
(more )
Specimen
5C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Shhtm1Chg/Shh+
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: prosencephalon |
Present |
Regionally restricted |
5C
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Expression in rostral midline of forebrain.
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Specimen
5D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: prosencephalon |
Absent |
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5D
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No expression in rostral prosencephalon.
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Specimen
5E:
embryonic day 9.5; Shhtm1Chg/Shh +
(more )
Specimen
5E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Shhtm1Chg/Shh+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future forebrain |
Present |
Regionally restricted |
5E
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Expression in rostral midline of forebrain.
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Analysis Tools
