Gene Expression Data
Assay Details
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Assay
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Reference:
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J:148194
Han DY, et al., Differential Islet-1 expression among lumbosacral spinal motor neurons in prenatal mouse. Brain Res. 2009 Apr 10;1265:30-6
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5699345
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Antibody:
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Anti-Islet1/2 (H120) sc-30200
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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The specificity of the antibody used was not detailed; both family members have been annotated.
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Results
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Specimen
2B:
embryonic day 12.5
(more  )
Specimen
2B:
(close  )
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Genetic Background:
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ICR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord lateral wall |
Present |
Regionally restricted |
2B
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Expressed in the medial motor column and the lateral motor column.
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Specimen
not shown:
embryonic day 13.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord lateral wall |
Present |
Regionally restricted |
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Expressed in motoneuron columns in the retrodorsolateral, dorsolateral, and ventral nucleus. Expressed in both the sympathetic preganglionic neurons and the parasympathetic preganglionic neurons within the intermediolateral column.
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Specimen
not shown:
embryonic day 14.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord lateral wall |
Present |
Regionally restricted |
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Expressed in motoneuron columns in the retrodorsolateral, dorsolateral, and ventral nucleus. Expressed in both the sympathetic preganglionic neurons and the parasympathetic preganglionic neurons within the intermediolateral column.
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Specimen
not shown:
embryonic day 15.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: ventral grey horn |
Strong |
Regionally restricted |
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Expressed in the sympathetic preganglionic neurons of the intermediolateral column.
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Specimen
not shown:
embryonic day 16.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: ventral grey horn |
Strong |
Regionally restricted |
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Expressed in the sympathetic preganglionic neurons of the intermediolateral column.
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Analysis Tools