Antibody:	anti-ChAT
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Clusters	2C	Expressed in the medial motor column and the lateral motor column.

Structure	Level	Pattern	Image	Note
TS21: spinal cord lateral wall	Present	Regionally restricted	3B	Expressed in motoneuron columns in the ventral horn of the rostral lumbar cord, whose locations are similar to the retrodorsolateral, dorsolateral, and ventral nucleus.

Structure	Level	Pattern	Image	Note
TS21: spinal cord lateral wall	Present	Regionally restricted	3D	Expressed in motoneuron columns in the ventral horn of the caudal lumbar cord, whose locations are similar to the retrodorsolateral, dorsolateral, and ventral nucleus.

Structure	Level	Pattern	Image	Note
TS21: spinal cord lateral wall	Present	Regionally restricted	3F	Expressed in motoneurons, parasympathetic and sympathetic preganglionic neurons in the ventral horn of the caudal-most lumbar and upper sacral cord, whose locations are similar to the retrodorsolateral, dorsolateral, and ventral nucleus.

Structure	Level	Pattern	Image	Note
TS22: spinal cord lateral wall	Present	Regionally restricted	4B	Expressed in motoneuron columns in the ventral horn of the rostral lumbar cord, whose locations are similar to the retrodorsolateral, dorsolateral, intermediolateral, and ventral nucleus.

Structure	Level	Pattern	Image	Note
TS22: spinal cord lateral wall	Present	Regionally restricted	4D	Expressed in motoneuron columns in the ventral horn of the caudal lumbar cord, whose locations are similar to the retrodorsolateral, dorsolateral, intermediolateral, and ventral nucleus.

Structure	Level	Pattern	Image	Note
TS22: spinal cord lateral wall	Present	Regionally restricted	4F	Expressed in motoneurons, parasympathetic and sympathetic preganglionic neurons in the ventral horn of the caudal-most lumbar and upper sacral cord, whose locations are similar to the retrodorsolateral, dorsolateral, intermediolateral and ventral nucleus.

Structure	Level	Pattern	Image	Note
TS23: ventral grey horn	Present	Regionally restricted	5B	Expressed in motoneuron columns in the ventral horn of the rostral lumbar cord, whose locations are similar to the retrodorsolateral, dorsolateral, intermediolateral, and ventral nucleus.

Structure	Level	Pattern	Image	Note
TS23: ventral grey horn	Present	Regionally restricted	5D	Expressed in motoneuron columns in the ventral horn of the caudal lumbar cord, whose locations are similar to the retrodorsolateral, dorsolateral, intermediolateral, and ventral nucleus.

Structure	Level	Pattern	Image	Note
TS23: ventral grey horn	Present	Regionally restricted	5F	Expressed in motoneurons, parasympathetic and sympathetic preganglionic neurons in the ventral horn of the caudal-most lumbar and upper sacral cord, whose locations are similar to the retrodorsolateral, dorsolateral, intermediolateral and ventral nucleus.

Structure	Level	Pattern	Image	Note
TS24: ventral grey horn	Present	Regionally restricted		Expressed in motoneurons, parasympathetic and sympathetic preganglionic neurons in the ventral horn, whose locations are similar to the retrodorsolateral, dorsolateral, intermediolateral and ventral nucleus.