Gene Expression Data
Assay Details
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Assay
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Reference:
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J:214586
Duan X, et al., Type II cadherins guide assembly of a direction-selective retinal circuit. Cell. 2014 Aug 14;158(4):793-807
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5699341
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Gene symbol:
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Cdh8
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Gene name:
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cadherin 8
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Reporter:
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lacZ
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Antibody:
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anti-B-galactosidase
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
3A:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Cdh8tm1Mta/Cdh8+
Neto1tm1.1(cre)Jrs/Neto1+
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Cdh8 reporter; red - Neto1 (assay MGI:5698645); blue - NeuroTrace Nissl (nuclei).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
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3A center
,
3A left
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer.
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| TS28: retina inner plexiform layer |
Present |
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3A center
,
3A left
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer.
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Specimen
4L:
postnatal day 6; Cdh8tm1Mta/Cdh8 +
(more  )
Note: Triple labeled: green - Cdh8 reporter; red - Slc18a3 (VAChT; assay MGI:5699349); blue - NeuroTrace Nissl (nuclei).
Specimen
4L:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Cdh8tm1Mta/Cdh8+
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Cdh8 reporter; red - Slc18a3 (VAChT; assay MGI:5699349); blue - NeuroTrace Nissl (nuclei).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
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4L
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer.
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| TS28: retina inner plexiform layer |
Present |
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4L
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer.
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Specimen
4N:
postnatal day 9; Cdh8tm1Mta/Cdh8 +
(more )
Note: Triple labeled: green - Cdh8 reporter; red - Slc18a3 (VAChT; assay MGI:5699349); blue - NeuroTrace Nissl (nuclei).
Specimen
4N:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Cdh8tm1Mta/Cdh8+
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Age:
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postnatal day 9
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Cdh8 reporter; red - Slc18a3 (VAChT; assay MGI:5699349); blue - NeuroTrace Nissl (nuclei).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
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4N
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer.
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| TS28: retina inner plexiform layer |
Present |
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4N
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer.
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Specimen
4P:
postnatal day 12; Cdh8tm1Mta/Cdh8 +
(more )
Note: Triple labeled: green - Cdh8 reporter; red - Slc18a3 (VAChT; assay MGI:5699349); blue - NeuroTrace Nissl (nuclei).
Specimen
4P:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Cdh8tm1Mta/Cdh8+
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Age:
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postnatal day 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Cdh8 reporter; red - Slc18a3 (VAChT; assay MGI:5699349); blue - NeuroTrace Nissl (nuclei).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
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4P
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer.
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| TS28: retina inner plexiform layer |
Present |
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4P
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer.
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Specimen
4R:
postnatal day 21; Cdh8tm1Mta/Cdh8 +
(more )
Note: Triple labeled: green - Cdh8 reporter; red - Slc18a3 (VAChT; assay MGI:5699349); blue - NeuroTrace Nissl (nuclei).
Specimen
4R:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Cdh8tm1Mta/Cdh8+
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Age:
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postnatal day 21
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Cdh8 reporter; red - Slc18a3 (VAChT; assay MGI:5699349); blue - NeuroTrace Nissl (nuclei).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
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4R
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer.
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| TS28: retina inner plexiform layer |
Present |
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4R
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer.
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Specimen
4M:
postnatal day 6; Cdh8tm1Mta/ Cdh8tm1Mta
(more )
Note: Triple labeled: green - Cdh8 reporter; red - Slc18a3 (VAChT; assay MGI:5699349); blue - NeuroTrace Nissl (nuclei).
Specimen
4M:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Cdh8tm1Mta/Cdh8tm1Mta
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Cdh8 reporter; red - Slc18a3 (VAChT; assay MGI:5699349); blue - NeuroTrace Nissl (nuclei).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
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4M
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer. In the mutant, the axons arborize in two sublaminae (instead of one).
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| TS28: retina inner plexiform layer |
Present |
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4M
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer. In the mutant, the axons arborize in two sublaminae (instead of one).
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Specimen
4O:
postnatal day 9; Cdh8tm1Mta/ Cdh8tm1Mta
(more )
Note: Triple labeled: green - Cdh8 reporter; red - Slc18a3 (VAChT; assay MGI:5699349); blue - NeuroTrace Nissl (nuclei).
Specimen
4O:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Cdh8tm1Mta/Cdh8tm1Mta
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Age:
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postnatal day 9
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Cdh8 reporter; red - Slc18a3 (VAChT; assay MGI:5699349); blue - NeuroTrace Nissl (nuclei).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
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4O
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer. In the mutant, the axons arborize in two sublaminae (instead of one).
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| TS28: retina inner plexiform layer |
Present |
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4O
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer. In the mutant, the axons arborize in two sublaminae (instead of one).
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Specimen
4Q:
postnatal day 12; Cdh8tm1Mta/ Cdh8tm1Mta
(more )
Note: Triple labeled: green - Cdh8 reporter; red - Slc18a3 (VAChT; assay MGI:5699349); blue - NeuroTrace Nissl (nuclei).
Specimen
4Q:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Cdh8tm1Mta/Cdh8tm1Mta
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Age:
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postnatal day 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
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Triple labeled: green - Cdh8 reporter; red - Slc18a3 (VAChT; assay MGI:5699349); blue - NeuroTrace Nissl (nuclei).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
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4Q
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer. In the mutant, the axons arborize in two sublaminae (instead of one).
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| TS28: retina inner plexiform layer |
Present |
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4Q
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer. In the mutant, the axons arborize in two sublaminae (instead of one).
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Specimen
4S:
postnatal day 21; Cdh8tm1Mta/ Cdh8tm1Mta
(more )
Note: Triple labeled: green - Cdh8 reporter; red - Slc18a3 (VAChT; assay MGI:5699349); blue - NeuroTrace Nissl (nuclei).
Specimen
4S:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Cdh8tm1Mta/Cdh8tm1Mta
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Age:
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postnatal day 21
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Cdh8 reporter; red - Slc18a3 (VAChT; assay MGI:5699349); blue - NeuroTrace Nissl (nuclei).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
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4S
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer. In the mutant, the axons arborize in two sublaminae (instead of one).
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| TS28: retina inner plexiform layer |
Present |
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4S
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Expression present in type 2 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer. In the mutant, the axons arborize in two sublaminae (instead of one).
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Analysis Tools