About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:146871 Sadakata H, et al., SAP-1 is a microvillus-specific protein tyrosine phosphatase that modulates intestinal tumorigenesis. Genes Cells. 2009 Mar;14(3):295-308
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5698999
Gene symbol: Ptprh
Gene name: protein tyrosine phosphatase receptor type H
Antibody: SAP-1 (clone 123)
Detection system: Secondary antibody
Results
Specimen 2A: postnatal week 6 (more )
Note: Double labeled: red - Ptprh; green - Ctnnb1 (assay MGI:5699001).
Structure Level Pattern Image Note
TS28: jejunum epithelium Present 2A SAP-1 top , 2A Merge top , 2A Merge bottom , 2A SAP-1 bottom Expression is at the apical surface.
Specimen 2B: postnatal week 6 (more )
Structure Level Pattern Image Note
TS28: jejunum epithelium Present 2B SAP-1 Expression is at the apical surface.
Specimen 2C top: postnatal (more )
Note: Double labeled: green - Ptprh; red - Nherf1 (assay MGI:5699010).
Structure Level Pattern Image Note
TS28: jejunum epithelium Present 2C SAP-1 top , 2C Merge top
Specimen 2C bottom: postnatal (more )
Note: Double labeled with rhodamine-conjugated phalloidin (red).
Structure Level Pattern Image Note
TS28: jejunum epithelium Present 2C Merge bottom , 2C SAP-1 bottom
Specimen 2D WT: postnatal (more )
Note: Immuno-Electron Microscopy. Specimens were fixed in 4% paraformaldehyde and 1% glutaraldehyde, thick sections were immunolabeled with gold and post-fixed with 1% OsO4 and 0.1% ferrocyanide and embedded in Epon. Ulthrathin sections were examined by the electron microscope.
Structure Level Pattern Image Note
TS28: jejunum epithelium Present 2D WT Expression is at the microvilli.
Specimen 2D KO: postnatal; Ptprhtm1.1Mato/Ptprhtm1.1Mato (more )
Note: Immuno-Electron Microscopy. Specimens were fixed in 4% paraformaldehyde and 1% glutaraldehyde, thick sections were immunolabeled with gold and post-fixed with 1% OsO4 and 0.1% ferrocyanide and embedded in Epon. Ulthrathin sections were examined by the electron microscope.
Structure Level Pattern Image Note
TS28: jejunum epithelium Absent 2D KO
Specimen 3A Jejunum: postnatal (more )
Note: Double labeled with phalloidin (green).
Structure Level Pattern Image Note
TS28: jejunum epithelium Present 3A SAP-1 Jejunum , 3A Merge Jejunum Expression is at the apical surface.
Specimen 3A Stomach: postnatal week 6 (more )
Note: Double labeled with phalloidin (green).
Structure Level Pattern Image Note
TS28: stomach epithelium Weak 3A Merge Stomach , 3A SAP-1 Stomach Expression is at the apical surface of epithelial cells.
Specimen 3B: postnatal week 6 (more )
Note: Double labeled with Ulex europaeus agglutinin (green).
Structure Level Pattern Image Note
TS28: ileum epithelium Present 3B SAP-1 , 3B Merge Expression is at the microvilli and is absent in goblet cells.
Specimen 3D E14.5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: intestine epithelium Absent 3D SAP-1 E14.5
Specimen 3D E16.5: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: intestine epithelium Weak 3D SAP-1 E16.5
Specimen 3D P1: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: intestine epithelium Present 3D SAP-1 P1
Specimen 3D P8: postnatal day 8 (more )
Structure Level Pattern Image Note
TS28: intestine epithelium Present 3D SAP-1 P8
Specimen 4A: postnatal week 6 (more )
Note: Double labeled: red - Ptprh; green - Ctnnb1 (assay MGI:5699001).
Structure Level Pattern Image Note
TS28: jejunum epithelium Present 4A b-catenin , 4A SAP-1
Specimen 4Ba,b: postnatal week 6 (more )
Note: Double labeled: red - Ptprh; green - Ctnnb1 (assay MGI:5699001).
Structure Level Pattern Image Note
TS28: jejunum epithelium Present Regionally restricted 4Bb , 4Ba Expression is at the villus tip and is absent at the crypts.
Specimen 4Bc,d: postnatal week 6 (more )
Note: Double labeled: red - Ptprh; green - Ctnnb1 (assay MGI:5699001).
Structure Level Pattern Image Note
TS28: jejunum epithelium Present Regionally restricted 4Bc , 4Bd Expression is at the villus tip and is absent at the crypts.
Specimen 5D WT: postnatal (more )
Note: Double labeled: red - Ptprh; green - Ctnnb1 (assay MGI:5699001).
Structure Level Pattern Image Note
TS28: jejunum epithelium Present 5D WT Expression is at the apical brush border.
Specimen 5D KO: postnatal; Ptprhtm1.1Mato/Ptprhtm1.1Mato (more )
Note: Double labeled: red - Ptprh; green - Ctnnb1 (assay MGI:5699001).
Structure Level Pattern Image Note
TS28: jejunum epithelium Absent 5D KO
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/30/2024
MGI 6.23 		The Jackson Laboratory