About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:211182 Deng M, et al., Comparative expression analysis of POU4F1, POU4F2 and ISL1 in developing mouse cochleovestibular ganglion neurons. Gene Expr Patterns. 2014 May;15(1):31-7
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5697723
Gene symbol: Pou4f2
Gene name: POU domain, class 4, transcription factor 2
Antibody: anti-Pou4f2 (C-13) sc-6026
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1B/C: embryonic day 9.5 (more )
Note: Double labeled: green - Pou4f2; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS15: facio-acoustic preganglion complex Present Single cells 1C , 1B Expressed in only a very few cochleovestibular ganglion cells, which coexpressed Pou4f1.
TS15: otic pit epithelium Absent 1C , 1B
Specimen 1E/F: embryonic day 10.5 (more )
Note: Double labeled: green - Pou4f2; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS17: acoustic ganglion Present 1E , 1F Increased expression. Expression started in the kernel of cochleovestibular ganglion cells and co-localized with Pou4f1 positive cells.
TS17: otocyst epithelium Absent 1F , 1E
Specimen 1H/I: embryonic day 11.5 (more )
Note: Double labeled: green - Pou4f2; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS19: acoustic ganglion Present Regionally restricted 1I , 1H Increased expression. Expression started in the kernel of cochleovestibular ganglion cells and co-localized with Pou4f1 positive cells.
TS19: inner ear epithelium Absent 1H , 1I
Specimen 1K/L: embryonic day 12.5 (more )
Note: Double labeled: green - Pou4f2; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS20: cochlear ganglion Present Regionally restricted 1K , 1L Expressed in a small group of cells near the cochlear duct.
TS20: vestibulo-cochlear ganglion Present Regionally restricted 1L , 1K Expressed in the center.
Specimen 1N/O: embryonic day 15.5 (more )
Note: Double labeled: green - Pou4f2; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS23: cochlear ganglion Present Widespread 1N , 1O Expression expanded to almost all spiral ganglion neurons, co-localizing with the Pou4f1-expressing cells.
Specimen 1Q/R: embryonic day 17.5 (more )
Note: Double labeled: green - Pou4f2; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS25: cochlear ganglion Strong 1R , 1Q
Specimen 1T/U: postnatal day 0 (more )
Note: Double labeled: green - Pou4f2; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS27: cochlear ganglion Strong 1T , 1U
Specimen 1W/X: postnatal day 0 (more )
Note: Double labeled: green - Pou4f2; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS27: vestibular ganglion Strong 1W , 1X
Specimen 4A/C: embryonic day 9.5 (more )
Note: Double labeled: green - Pou4f2; red - Neurod1 (assay MGI:5697839).
Structure Level Pattern Image Note
TS15: facio-acoustic preganglion complex Present Single cells 4C , 4A Only expressed in Neurod1-negative, differentiated neural cochleovestibular ganglion cells.
Specimen 4D/F: embryonic day 10.5 (more )
Note: Double labeled: green - Pou4f2; red - Neurod1 (assay MGI:5697839).
Structure Level Pattern Image Note
TS17: acoustic ganglion Present Regionally restricted 4D , 4F Only expressed in Neurod1-negative, differentiated neural cochleovestibular ganglion cells.
Specimen 4G/I: embryonic day 11.5 (more )
Note: Double labeled: green - Pou4f2; red - Neurod1 (assay MGI:5697839).
Structure Level Pattern Image Note
TS19: acoustic ganglion Present Regionally restricted 4G , 4I Only expressed in Neurod1-negative, differentiated neural cochleovestibular ganglion cells.
Specimen 4M/O: embryonic day 11.5 (more )
Note: Double labeled: green - Pou4f2; red - BrdU.
Structure Level Pattern Image Note
TS19: acoustic ganglion Present Regionally restricted 4O , 4M Not expressed in proliferating cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory