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Gene Expression Data
Assay Details
Assay
Reference: J:220858 Kim JY, et al., Common and distinctive localization patterns of Crumbs polarity complex proteins in the mammalian eye. Gene Expr Patterns. 2015 Jan;17(1):31-7
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5697431
Gene symbol: Pals1
Gene name: protein associated with LIN7 1, MAGUK family member
Antibody: Pals1 antibody (07-708)
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 2A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: retina Present Regionally restricted 2A Expression is at the apical surface of the optic cup.
TS20: lens vesicle epithelium Present 2A Expression is at the apical surface.
Specimen 3A: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: retina Present Punctate 3A Widespread punctate staining in the inner retina.
Specimen 3B: embryonic day 16.5 (more )
Note: (E,H) are higher magnifications of the boxed areas in (B). Double labeled: red - Pals1; green - Cdh2 (assay MGI:5697432).
Structure Level Pattern Image Note
TS24: retina Present Regionally restricted 3E , 3B , 3E2 Expression is at the apical surface of the retina.
TS24: retina nuclear layer Present Regionally restricted 3H2 , 3B , 3H Expression is in the cytoplasm of retinal cells as well as in apical junctions of most retinal cells in the outer and inner neuroblastic layers.
Specimen 3C: embryonic day 19.5 (more )
Structure Level Pattern Image Note
TS26: retina Present Regionally restricted 3C Expression is at the apical surface of the retinal epithelium.
Specimen 3D: postnatal day 21 (more )
Structure Level Pattern Image Note
TS28: retina Present Regionally restricted 3D Expression is at the apical surface of the retinal epithelium.
Specimen 3F: embryonic day 16.5 (more )
Note: Double labeled: red - Pals1; green - Prkci (assay MGI:5697433) and Prkcz (assay MGI:5697519).
Structure Level Pattern Image Note
TS24: retina Present 3F , 3F2
Specimen 3G: postnatal day 2 (more )
Note: Double labeled: red - Pals1; green - Ezr (assay MGI:5697434).
Structure Level Pattern Image Note
TS27: retina Present 3G , 3G2
Specimen 4A: embryonic day 14.5 (more )
Note: (A1,C1) are higher magnifications of the boxed areaa in (A,C). Double labeled: red - Pals1; green - Golga2 (assay MGI:5697453).
Structure Level Pattern Image Note
TS22: retina Present Punctate 4A , 4C , 4A1 , 4C1
Specimen 4G: postnatal day 10 (more )
Note: Double labeled: red - Pals1; green - Golga2 (assay MGI:5697453).
Structure Level Pattern Image Note
TS28: photoreceptor layer Present 4G2 , 4G
Specimen 4H: postnatal day 21 (more )
Note: Double labeled: red - Pals1; green - Golga2 (assay MGI:5697453).
Structure Level Pattern Image Note
TS28: photoreceptor layer Present 4H , 4H2
Specimen 6A: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: lens epithelium Present 6A Expression is at the apical surface and at subapical areas in the vicinity of nuclei.
Specimen 6D: embryonic day 14.5 (more )
Note: Double labeled: red - Pals1; green - Golga2 (assay MGI:5697453).
Structure Level Pattern Image Note
TS22: lens epithelium Present 6D , 6D2 Expression is at the apical surface and at subapical areas in the vicinity of nuclei.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory