Probe:	M-twist cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	The authors state that identical results were obtained with a 3' and 5' probe and did not specify which one was used in the experiment.

Structure	Level	Pattern	Image	Note
TS8: embryo	Absent

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present	Regionally restricted	1A	Expression is lateral to the primitive streak.
TS10: allantois	Present		1A
TS10: primitive streak	Absent		1A

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present	Regionally restricted	1B	Expression is lateral to the primitive streak.
TS10: allantois	Present		1B
TS10: primitive streak	Absent		1B

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present	Regionally restricted	1C	Expression is lateral to the primitive streak and is strong in the anterior part.
TS10: primitive streak	Absent		1C

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present	Regionally restricted	1D	Expression is lateral to the primitive streak.
TS10: allantois	Present		1D
TS10: primitive streak	Absent		1D

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Regionally restricted	2A	Expression is in the primordium of the thoracic body wall at the level of the foregut and heart.
TS12: allantois	Present		2A
TS12: head mesenchyme	Present		2A
TS12: somatopleure	Present		2A
TS12: somite	Present		2A
TS12: cardiac muscle tissue	Absent		2A
TS12: notochord	Absent		2A

Structure	Level	Pattern	Image	Note
TS12: somite	Present		2B	Expression is in somites and in the forming somite.
TS12: trunk unsegmented mesenchyme	Present	Regionally restricted	2B	Expression is in the region of the next forming somite.
TS12: allantois	Present		2B
TS12: notochord	Absent		2B

Structure	Level	Pattern	Image	Note
TS12: somatopleure	Very strong		2C
TS12: somite	Very strong		2C
TS12: embryo ectoderm	Absent		2C
TS12: embryo endoderm	Absent		2C
TS12: splanchnopleure	Absent		2C

Structure	Level	Pattern	Image	Note
TS12: trunk unsegmented mesenchyme	Present	Regionally restricted	2D	Expression is in the region of the next forming somite (Figure 2D shows an area that is neagtive for expression).

Structure	Level	Pattern	Image	Note
TS12: head mesenchyme	Present	Single cells	2E
TS12: neural ectoderm	Absent		2E

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch	Strong		3A
TS13: head mesenchyme	Present		3A
TS13: lateral plate mesoderm	Present		3A
TS13: optic eminence mesenchyme	Present		3A
TS13: somite	Present		3A

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch	Strong		3B
TS13: optic eminence mesenchyme	Present		3B

Structure	Level	Pattern	Image	Note
TS13: somite	Present		3C	Expression in the anterior somites is in the medial ventral half and in posterior somites there is an uniform distribution.
TS13: somatopleure	Present		3C
TS13: splanchnopleure	Absent		3C

Structure	Level	Pattern	Image	Note
TS15: dermomyotome	Present
TS15: sclerotome	Present
TS15: myotome	Absent

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Strong		4A
TS17: 1st branchial arch maxillary component	Strong		4A
TS17: skeleton	Present	Regionally restricted	4A	Expression is in occipital bone and facial bone primordia.
TS17: 1st branchial arch	Present		4A
TS17: 2nd branchial arch	Present		4A
TS17: head mesenchyme	Present		4A
TS17: limb	Present		4A
TS17: somite	Present		4A
TS17: 3rd branchial arch	Weak		4A
TS17: otocyst	Absent		4A

Structure	Level	Pattern	Image	Note
TS17: dermomyotome	Present		4B
TS17: sclerotome	Present		4B
TS17: myotome	Absent		4B
TS17: neural tube	Absent		4B

Structure	Level	Pattern	Image	Note
TS20: limb interdigital region	Very strong		5A
TS20: ear	Strong		5A
TS20: face	Strong		5A
TS20: limb	Strong		5A
TS20: tail somite	Strong		5A
TS20: embryo mesenchyme	Present	Regionally restricted	5A	Expression is in the mesenchyme under the epidermis.

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Regionally restricted	5B	Expression is around cartilage and muscle anlagen.
TS20: tongue mesenchyme	Present		5B

Structure	Level	Pattern	Image	Note
TS20: embryo	Present		5C	Expression is around cartilage and muscle anlagen.
TS20: heart	Present	Regionally restricted	5C	Expression is in heart valves including the aortic valve.
TS20: forelimb pre-cartilage condensation	Present	Graded	5C	Expression is strongest close to the areas of chondrogenic differentiation and decreases with distance.
TS20: atrioventricular endocardial cushion	Present		5C
TS20: tail	Present		5C

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Regionally restricted	5D	Expression is around cartilage and muscle anlagen.
TS20: diaphragm	Present	Regionally restricted	5D	Expression is in the caudal extremity of the diaphragm.
TS20: genital tubercle	Present		5D
TS20: tail	Present		5D
TS20: hindlimb	Present	Graded	5D	Expression is strongest close to the areas of chondrogenic differentiation and decreases with distance.

Structure	Level	Pattern	Image	Note
TS19: vertebral pre-cartilage condensation	Present		6A

Structure	Level	Pattern	Image	Note
TS22: atrioventricular endocardial cushion	Present		7A

Structure	Level	Pattern	Image	Note
TS22: mammary mesenchyme	Strong		7B

Structure	Level	Pattern	Image	Note
TS22: molar mesenchyme	Strong		7C

Structure	Level	Pattern	Image	Note
TS26: face mesenchyme	Strong	Regionally restricted	8A	Expression is in the mesenchyme surrounding the follicles of the whiskers and underneath the epidermis.
TS26: vibrissa dermal component	Strong		8A	Expression is in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS26: molar mesenchyme	Strong		8B