Antibody:	mAb F22
Detection system:	Secondary antibody coupled to rhodamine
Assay notes:	The antibody recognizes two proteins; both have been annotated.

Structure	Level	Pattern	Image	Note
TS27: retina	Absent		1a

Structure	Level	Pattern	Image	Note
TS27: photoreceptor layer	Weak	Regionally restricted	1b	Expressed around the photoreceptor inner segments.

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer	Strong	Regionally restricted	1c	Expressed in the interphotoreceptor matrix.

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Strong	Regionally restricted	1d	Expressed in the interphotoreceptor matrix around the outer segments. A sharp reduction in expression at the border between outer and inner segments. There was no significant variation in expression distribution or strength.
TS28: pigmented retinal epithelium	Present	Patchy	1d
TS28: photoreceptor layer inner segment	Absent		1d

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Strong	Uniform	1e	Evenly distributed expression around rod and cone outer segments. There was no significant variation in expression distribution or strength.
TS28: photoreceptor layer inner segment	Weak	Punctate	1e	Expressed intermittently at the inner segment level around individual photoreceptors.

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Present	Uniform	1f	Expressed around rod and cone outer segments. Slightly less expression than at P13. There was no significant variation in expression distribution or strength.
TS28: photoreceptor layer inner segment	Present		1f	Slightly less expression than at P13.

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Present	Uniform	1g	Expressed around rod and cone outer segments. Slightly less expression than at P13. There was no significant variation in expression distribution or strength.
TS28: photoreceptor layer inner segment	Present		1g	Expressed around individual photoreceptors at the inner segment level.

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Present	Non-Uniform	1h	Drastic reduction in expression evenly throughout the layer. Streaks of stronger labeling are seen along the length of the outer segments.
TS28: photoreceptor layer inner segment	Weak		1h

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Present	Non-Uniform	1i	Some individual photoreceptors have a stronger label intensity in their interphotoreceptor matrix domain than the others.
TS28: photoreceptor layer inner segment	Weak		1i

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer	Strong	Regionally restricted	1j	Individual cone photoreceptors are surrounded with a strong and defined expression domain, extending from the apical side of the retinal pigment epithelium to the scleral half of the inner segment.

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer	Strong	Regionally restricted	1k	Expression around rods is completely abolished and only the cone domains are strongly positive.
TS28: pigmented retinal epithelium	Present	Regionally restricted	1k	Expressed intermittently.

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer	Strong	Regionally restricted	1l	Expression around rods is completely abolished and only the cone domains are strongly positive.
TS28: pigmented retinal epithelium	Present	Regionally restricted	1l	Expressed intermittently.

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Present	Regionally restricted	2b	Expressed only in the cone outer segments (marked by cone-specific antibodies in tangential section).

Structure	Level	Pattern	Image	Note
TS20: eye	Absent		3a

Structure	Level	Pattern	Image	Note
TS19: eye	Absent

Structure	Level	Pattern	Image	Note
TS21: eye	Absent

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer	Strong	Regionally restricted		Expression around rods is completely abolished and only the cone domains are strongly positive.
TS28: pigmented retinal epithelium	Present	Regionally restricted		Expressed intermittently.
TS28: retina inner nuclear layer	Absent
TS28: retina inner plexiform layer	Absent
TS28: retina limiting lamina	Absent
TS28: vitreous humour	Absent

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Absent
TS28: retina inner plexiform layer	Absent
TS28: retina limiting lamina	Absent
TS28: vitreous humour	Absent

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer	Present	Regionally restricted		Only expressed in the cone matrix domain.