Antibody:	anti-PS-1
Assay notes:	The antibody used in this assay recognizes the phosphorylated form of the protein translated from three genes. RT-PCR experiments presented in this paper suggest that all 3 genes are transcribed at this time, so all three family members have been annotated when appropriate.

Structure	Level	Pattern	Image	Note
TS8: epiblast	Present	Regionally restricted	5A	Expression is seen in the proximal region.
TS8: primitive endoderm	Present		5A	Expression is seen in the visceral endoderm.
TS8: extraembryonic ectoderm	Absent		5A

Structure	Level	Pattern	Image	Note
TS8: epiblast	Present	Regionally restricted	5B	Expression is seen in the proximal region.
TS8: primitive endoderm	Present		5B	Expression is seen in the visceral endoderm.
TS8: extraembryonic ectoderm	Absent		5B

Structure	Level	Pattern	Image	Note
TS9: embryo	Present	Regionally restricted	5C	Expression is seen in the proximal region of the epiblast.
TS9: primitive endoderm	Absent		5C	No expression is seen in the visceral endoderm.
TS9: extraembryonic ectoderm	Absent		5C

Structure	Level	Pattern	Image	Note
TS9: embryo	Present	Regionally restricted	5D	Expression is seen in the proximal region of the epiblast.
TS9: primitive endoderm	Absent		5D	No expression is seen in the visceral endoderm.
TS9: extraembryonic ectoderm	Absent		5D

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	5E	Expression is seen in the proximal region of the epiblast.
TS10: primitive endoderm	Absent		5E	No expression is seen in the visceral endoderm.
TS10: extraembryonic ectoderm	Absent		5E

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	5F	Expression is seen in the proximal region of the epiblast.
TS10: primitive endoderm	Absent		5F	No expression is seen in the visceral endoderm.
TS10: extraembryonic ectoderm	Absent		5F

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	7D	Expression is localized in the proximal region of the epiblast.