Gene Expression Data
Assay Details
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Assay
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Reference:
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J:79513
Hayashi K, et al., SMAD1 signaling is critical for initial commitment of germ cell lineage from mouse epiblast. Mech Dev. 2002 Oct;118(1-2):99
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5696295
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Gene symbol:
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Smad1
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Gene name:
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SMAD family member 1
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
not shown:
embryonic day 3.5; Smad1tm1Tako/Smad1 +
(more  )
Note: blastocyst stage
Specimen
not shown:
(close  )
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Genetic Background:
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involves: 129S/SvEv * 129P2/OlaHsd * C57BL/6 * DBA/2 * ICR
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Mutant Allele(s):
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Smad1tm1Tako/Smad1+
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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blastocyst stage
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Specimen
4A:
embryonic day 5.5; Smad1tm1Tako/Smad1 +
(more )
Note: pre-gastrulation stage
Specimen
4A:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129P2/OlaHsd * C57BL/6 * DBA/2 * ICR
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Mutant Allele(s):
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Smad1tm1Tako/Smad1+
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Age:
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embryonic day 5.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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pre-gastrulation stage
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Specimen
4B:
embryonic day 6.0; Smad1tm1Tako/Smad1 +
(more )
Note: mid-streak stage
Specimen
4B:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129P2/OlaHsd * C57BL/6 * DBA/2 * ICR
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Mutant Allele(s):
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Smad1tm1Tako/Smad1+
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Age:
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embryonic day 6.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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mid-streak stage
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Specimen
4C:
embryonic day 6.5; Smad1tm1Tako/Smad1 +
(more )
Note: late streak stage
Specimen
4C:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129P2/OlaHsd * C57BL/6 * DBA/2 * ICR
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Mutant Allele(s):
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Smad1tm1Tako/Smad1+
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Age:
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embryonic day 6.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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late streak stage
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Specimen
4D:
embryonic day 7.25; Smad1tm1Tako/Smad1 +
(more )
Note: early bud stage
Specimen
4D:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129P2/OlaHsd * C57BL/6 * DBA/2 * ICR
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Mutant Allele(s):
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Smad1tm1Tako/Smad1+
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Age:
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embryonic day 7.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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early bud stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo mesoderm |
Present |
Regionally restricted |
4D
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Expression is seen in the proximal region.
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| TS10: allantois |
Present |
Regionally restricted |
4D
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Expression is seen at the base.
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Specimen
4E:
embryonic day 7.5; Smad1tm1Tako/Smad1 +
(more )
Note: neural plate stage
Specimen
4E:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129P2/OlaHsd * C57BL/6 * DBA/2 * ICR
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Mutant Allele(s):
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Smad1tm1Tako/Smad1+
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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neural plate stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo mesoderm |
Present |
Regionally restricted |
4E
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Expression is seen in the proximal region.
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| TS11: allantois |
Present |
Regionally restricted |
4E
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Expression is seen at the base.
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Specimen
4F:
embryonic day 7.5-8.0; Smad1tm1Tako/Smad1 +
(more )
Note: headfold stage
Specimen
4F:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129P2/OlaHsd * C57BL/6 * DBA/2 * ICR
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Mutant Allele(s):
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Smad1tm1Tako/Smad1+
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Age:
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embryonic day 7.5-8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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headfold stage
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Specimen
4G:
embryonic day 5.5; Smad1tm1Tako/Smad1 +
(more )
Note: pre-gastrulation stage
Specimen
4G:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129P2/OlaHsd * C57BL/6 * DBA/2 * ICR
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Mutant Allele(s):
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Smad1tm1Tako/Smad1+
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Age:
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embryonic day 5.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Note:
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pre-gastrulation stage
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Specimen
4H:
embryonic day 5.5; Smad1tm1Tako/Smad1 +
(more )
Note: pre-gastrulation stage
Specimen
4H:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129P2/OlaHsd * C57BL/6 * DBA/2 * ICR
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Mutant Allele(s):
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Smad1tm1Tako/Smad1+
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Age:
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embryonic day 5.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Note:
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pre-gastrulation stage
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Specimen
4I:
embryonic day 5.75; Smad1tm1Tako/Smad1 +
(more )
Note: early streak stage
Specimen
4I:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129P2/OlaHsd * C57BL/6 * DBA/2 * ICR
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Mutant Allele(s):
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Smad1tm1Tako/Smad1+
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Age:
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embryonic day 5.75
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Note:
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early streak stage
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Specimen
4J:
embryonic day 6.0; Smad1tm1Tako/Smad1 +
(more )
Note: mid-streak stage
Specimen
4J:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129P2/OlaHsd * C57BL/6 * DBA/2 * ICR
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Mutant Allele(s):
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Smad1tm1Tako/Smad1+
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Age:
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embryonic day 6.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Note:
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mid-streak stage
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Specimen
4K:
embryonic day 7.25; Smad1tm1Tako/Smad1 +
(more )
Note: early bud stage
Specimen
4K:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129P2/OlaHsd * C57BL/6 * DBA/2 * ICR
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Mutant Allele(s):
|
Smad1tm1Tako/Smad1+
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Age:
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embryonic day 7.25
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Note:
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early bud stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo mesoderm |
Present |
Regionally restricted |
4K
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Expression is seen in the proximal region.
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Specimen
4L:
embryonic day 7.5-8.0; Smad1tm1Tako/Smad1 +
(more )
Note: headfold stage
Specimen
4L:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129P2/OlaHsd * C57BL/6 * DBA/2 * ICR
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Mutant Allele(s):
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Smad1tm1Tako/Smad1+
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Age:
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embryonic day 7.5-8.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Note:
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headfold stage
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Specimen
4M:
embryonic day 7.25; Smad1tm1Tako/Smad1 +
(more )
Note: early bud stage; the section, which is at the level of the base of the allantois, is also stained for alkaline phosphatase activity (brown). Embedded in methacrylate.
Specimen
4M:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129P2/OlaHsd * C57BL/6 * DBA/2 * ICR
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Mutant Allele(s):
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Smad1tm1Tako/Smad1+
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Age:
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embryonic day 7.25
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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early bud stage; the section, which is at the level of the base of the allantois, is also stained for alkaline phosphatase activity (brown). Embedded in methacrylate.
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Specimen
4N:
embryonic day 7.5-8.0; Smad1tm1Tako/Smad1 +
(more )
Note: headfold stage; the section, which is at the level of the base of the allantois, is also stained for alkaline phosphatase activity (brown). Embedded in methacrylate.
Specimen
4N:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129P2/OlaHsd * C57BL/6 * DBA/2 * ICR
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Mutant Allele(s):
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Smad1tm1Tako/Smad1+
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Age:
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embryonic day 7.5-8.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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headfold stage; the section, which is at the level of the base of the allantois, is also stained for alkaline phosphatase activity (brown). Embedded in methacrylate.
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Specimen
4O:
embryonic day 8.5; Smad1tm1Tako/Smad1 +
(more )
Note: early somite stage; the section, which is at the level of the base of the allantois, is also stained for alkaline phosphatase activity (brown). Embedded in methacrylate.
Specimen
4O:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129P2/OlaHsd * C57BL/6 * DBA/2 * ICR
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Mutant Allele(s):
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Smad1tm1Tako/Smad1+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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early somite stage; the section, which is at the level of the base of the allantois, is also stained for alkaline phosphatase activity (brown). Embedded in methacrylate.
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