Antibody:	anti-Phospho-MAP Kinase (Thr183/Tyr185) (M8159)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Two antibodies were used for this study. Authors state the rabbit polyclonal ( MGI:2677398) was used in a majority of cases, but a mouse monoclonal ( MGI:3578364) may have been used. Both antibodies recognize more than one gene (Mapk1 andMapk3); both have been annotated.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	1G	In the central nervous system, expression first observed in the neural ectoderm of the anterior forebrain.
TS13: midbrain-hindbrain junction	Present		1G	Expression across the midbrain-hindbrain boundary.
TS13: foregut diverticulum	Present		1G	Sustained expression in foregut.
TS13: sinus venosus	Present		1G	Transient expression in sinus venosus.

Structure	Level	Pattern	Image	Note
TS14: dorsal aorta	Present		1H	Prevalent expression in dorsal aorta.
TS14: intersomitic artery	Present		1H	Expression in newly forming intersomitic vessels sprouting from the dorsal aorta.
TS14: branchial arch	Present		1H	Sustained expression in frontonasal process, midbrain-hindbrain junction, branchial arches, foregut, limb bud, and tail bud.
TS14: foregut	Present		1H	Sustained expression in frontonasal process, midbrain-hindbrain junction, branchial arches, foregut, limb bud, and tail bud.
TS14: fronto-nasal process	Present		1H	Sustained expression in frontonasal process, midbrain-hindbrain junction, branchial arches, foregut, limb bud, and tail bud.
TS14: limb bud	Present		1H	Sustained expression in frontonasal process, midbrain-hindbrain junction, branchial arches, foregut, limb bud, and tail bud.
TS14: midbrain-hindbrain junction	Present		1H	Sustained expression in frontonasal process, midbrain-hindbrain junction, branchial arches, foregut, limb bud, and tail bud.
TS14: tail bud	Present		1H	Sustained expression in frontonasal process, midbrain-hindbrain junction, branchial arches, foregut, limb bud, and tail bud.
TS14: ear	Present		1H	Transient expression in ear primordia.

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		1I	Sustained expression in frontonasal process, forebrain, midbrain-hindbrain junction, branchial arches, forelimb bud, hindlimb bud, and tail bud.
TS15: forelimb bud	Present		1I	Sustained expression in frontonasal process, forebrain, midbrain-hindbrain junction, branchial arches, forelimb bud, hindlimb bud, and tail bud.
TS15: fronto-nasal process	Present		1I	Sustained expression in frontonasal process, forebrain, midbrain-hindbrain junction, branchial arches, forelimb bud, hindlimb bud, and tail bud.
TS15: hindlimb ridge	Present		1I	Sustained expression in frontonasal process, forebrain, midbrain-hindbrain junction, branchial arches, forelimb bud, hindlimb bud, and tail bud.
TS15: midbrain-hindbrain junction	Present		1I	Sustained expression in frontonasal process, forebrain, midbrain-hindbrain junction, branchial arches, forelimb bud, hindlimb bud, and tail bud.
TS15: tail	Present		1I	Sustained expression in frontonasal process, forebrain, midbrain-hindbrain junction, branchial arches, forelimb bud, hindlimb bud, and tail bud.
TS15: ear	Present		1I	Transient expression in eye primordia and ear primordia.
TS15: eye	Present		1I	Transient expression in eye primordia and ear primordia.
TS15: somite	Present	Regionally restricted	1I	Transient expression in caudal region of somites.
TS15: intersomitic artery	Present	Regionally restricted	1I	Expression only in nascent blood vesels growing between the most caudal somites.

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present		1J	Sustained expression in frontonasal process, branchial arches and limb bud.
TS17: fronto-nasal process	Present		1J	Sustained expression in frontonasal process, branchial arches and limb bud.
TS17: limb bud	Present		1J	Sustained expression in frontonasal process, branchial arches and limb bud.
TS17: ganglion	Present		1J	Transient expression in nasal pits and ganglia.
TS17: nasal pit	Present		1J	Transient expression in nasal pits and ganglia.

Structure	Level	Pattern	Image	Note
TS14: migrating neural crest	Present		3B , 3A	Prevalent expression in neural crest cells migrating from the neural tube.

Structure	Level	Pattern	Image	Note
TS14: migrating neural crest	Present		3C	Prevalent expression in neural crest cells migrating from the neural tube.

Structure	Level	Pattern	Image	Note
TS17: glossopharyngeal ganglion	Present	Regionally restricted	3D	Expression in nerve tracts of glossopharyngeal and vagus ganglia.
TS17: vagus ganglion	Present	Regionally restricted	3D	Expression in nerve tracts of glossopharyngeal and vagus ganglia.

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		3F , 3E	Expression in dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS17: spinal nerve	Present	Regionally restricted	3H , 3G	Expression in sensory nerve root along the length of the neural tube.

Structure	Level	Pattern	Image	Note
TS15: hindlimb ridge ectoderm	Weak		7E	As limb bud outgrowth continues, expression in surface ectoderm is diminished.
TS15: hindlimb ridge mesenchyme	Trace		7E	Expression in underlying mesenchyme is dramatically increased.

Structure	Level	Pattern	Image	Note
TS16: hindlimb bud ectoderm	Present	Graded	7F	Pronounced gradient of expression in surface ectodermal cells with intensity strongest adjacent to the apical ectodermal ridge. Expression in some regions of the dorsal and ventral surface ectoderm.
TS16: hindlimb bud mesenchyme	Present	Graded	7F	Gradient of expression in the mesenchyme beneath the surface ectoderm in some ventral regions.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud ectoderm	Present	Graded	7G	Pronounced gradient of expression in surface ectodermal cells with intensity strongest adjacent to the apical ectodermal ridge. Expression in some regions of the dorsal and ventral surface ectoderm.
TS17: hindlimb bud mesenchyme	Present	Graded	7G	Gradient of expression in the mesenchyme beneath the surface ectoderm in some dorsal regions.