Antibody:	anti-Phospho-MAP Kinase (Thr183/Tyr185) (M8159)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to Cy3
Assay notes:	Two antibodies were used for this study. Authors state the rabbit polyclonal ( MGI:2677398) was used in a majority of cases, but a mouse monoclonal ( MGI:3578364) may have been used. Both antibodies recognize more than one gene (Mapk1 andMapk3); both have been annotated.

Structure	Level	Pattern	Image	Note
TS7: egg cylinder	Absent		1A

Structure	Level	Pattern	Image	Note
TS8: extraembryonic ectoderm	Present	Widespread	1B	Expression first observed throughout the extraembryonic ectoderm.
TS8: ectoplacental cone	Present	Widespread	1B	Expression throughout the ectoplacental cone began shortly after its formation.
TS8: epiblast	Present	Regionally restricted	1B

Structure	Level	Pattern	Image	Note
TS9: extraembryonic ectoderm	Present	Regionally restricted	1C	Expression became restricted to a ring in the most proximal region of the extraembryonic ectoderm.
TS9: ectoplacental cone	Present	Regionally restricted	1C	Expression in the ectoplacental cone was sustained in the central diploid population of ectoplacental cone cells.
TS9: embryo ectoderm	Present	Scattered	1C	Scattered expression in mitotic cells in some regions of embryonic ectoderm and mesoderm throughout development.
TS9: embryo mesoderm	Present	Scattered	1C	Scattered expression in mitotic cells in some regions of embryonic ectoderm and mesoderm throughout development.

Structure	Level	Pattern	Image	Note
TS10: extraembryonic ectoderm	Present	Regionally restricted	1D	Expression became restricted to a ring in the most proximal region of the extraembryonic ectoderm.
TS10: ectoplacental cone	Present	Regionally restricted	1D	Expression in the ectoplacental cone was sustained in the cental diploid population of ectoplacental cone cells.
TS10: allantois	Present		1D	Transient expression in the allantoic bud.
TS10: yolk sac mesoderm	Present	Regionally restricted	1D	Transient expression in blood island mesoderm.
TS10: embryo ectoderm	Present	Scattered	1D	Scattered expression in mitotic cells in some regions of embryonic ectoderm and mesoderm throughout development.
TS10: embryo mesoderm	Present	Scattered	1D	Scattered expression in mitotic cells in some regions of embryonic ectoderm and mesoderm throughout development.

Structure	Level	Pattern	Image	Note
TS11: extraembryonic ectoderm	Present	Regionally restricted	1E	Expression became restricted to a ring in the most proximal region of the extraembryonic ectoderm.
TS11: trophectoderm	Present	Regionally restricted	1E	Expression in the ectoplacental cone was sustained in the cental diploid population of ectoplacental cone cells.
TS11: head mesenchyme	Present		1E	Transient expression in the headfold mesoderm.

Structure	Level	Pattern	Image	Note
TS12: extraembryonic ectoderm	Weak	Regionally restricted	1F	Expression domain in the extraembryonic ectoderm decreased in intensity and cellular depth, and was barely detectable after 8.0 dpc.
TS12: ectoplacental cone	Present	Regionally restricted	1F	Expression in the ectoplacental cone was sustained in the cental diploid population of ectoplacental cone cells.
TS12: heart	Present		1F	Transient expression in the heart primordium.

Structure	Level	Pattern	Image	Note
TS14: dorsal aorta	Present		2A	Prevalent expression in dorsal aorta.
TS14: intersomitic artery	Present		2A

Structure	Level	Pattern	Image	Note
TS15: intersomitic artery	Present	Regionally restricted	2B	Expression in intersomitic vessels growing between the most caudal somites.

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		2C	Prevalent expression in dorsal aorta.
TS15: intersomitic artery	Present	Regionally restricted	2C	Expression in newly forming intersomitic vessels sprouting from the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS14: intersomitic artery	Present		2D	Expression in newly forming intersomitic vessels sprouting from the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS14: intersomitic artery	Present		2E	Expression in newly forming intersomitic vessels sprouting from the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS16: intersomitic artery	Present	Regionally restricted	2F	Expression intersomitic vessels growing between the most caudal somites.

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present	Regionally restricted	6A	Expression in contiguous domains in limb bud mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present	Regionally restricted	6B	Expression appeared cytoplasmic.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	6E	Expression in contiguous domains in forebrain neural ectoderm.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	6F	Expression appeared cytoplasmic.

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present	Regionally restricted	6M	Expression scattered along the apical side of embryo ectoderm.

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present	Punctate	6N	Dynamic, punctate expression.

Structure	Level	Pattern	Image	Note
TS14: forelimb bud ectoderm	Present	Regionally restricted	7A	Expression first observed during limb bud initiation in the surface ectoderm overlying lateral plate.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present	Regionally restricted	7B	Pronounced gradient of expression in mesenchymal cells with intensity strongest adjacent to the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present	Regionally restricted	7C	Pronounced gradient of expression in mesenchymal cells with intensity strongest adjacent to the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	7D	Pronounced gradient of expression in mesenchymal cells with intensity strongest adjacent to the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS7: extraembryonic ectoderm	Present	Graded	8A	Expression first seen as a gradient throughout entire extraembryonic ectoderm at 5.5 dpc.

Structure	Level	Pattern	Image	Note
TS8: extraembryonic ectoderm	Present	Regionally restricted	8B	Reduced range of expression; domain restricted to a ring of 6-9 cell diameters across.

Structure	Level	Pattern	Image	Note
TS10: extraembryonic ectoderm	Present	Regionally restricted	8C	Sharpened upper boundary of expression. Band of expression reduced to 4-6 cell diameters.

Structure	Level	Pattern	Image	Note
TS11: extraembryonic ectoderm	Present	Regionally restricted	8D	Ring of expression reducded to 2-4 cell diameters.

Structure	Level	Pattern	Image	Note
TS11: extraembryonic ectoderm	Present	Regionally restricted	8E	Expression in both peripheral and internal layers of extraembryonic ectoderm.