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Gene Expression Data
Assay Details
Assay
Reference: J:215153 Follit JA, et al., Arf4 is required for Mammalian development but dispensable for ciliary assembly. PLoS Genet. 2014 Feb;10(2):e1004170
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5695204
Gene symbol: Arf4
Gene name: ADP-ribosylation factor 4
Reporter: lacZ
Direct Detection
Assay notes: Fixative: 0.2% glutaraldehyde, 2% formalin.
Results
Specimen 5A +/-: embryonic day 7.5; Arf4Gt(AY0614)Wtsi/Arf4+ (more )
Structure Level Pattern Image Note
TS11: parietal endoderm Weak 5A +/-
TS11: extraembryonic visceral endoderm Strong 5A +/-

Specimen 5A -/-: embryonic day 7.5; Arf4Gt(AY0614)Wtsi/Arf4Gt(AY0614)Wtsi (more )
Structure Level Pattern Image Note
TS11: parietal endoderm Weak 5A -/-
TS11: extraembryonic visceral endoderm Strong 5A -/-

Specimen 5B: embryonic day 7.5; Arf4Gt(AY0614)Wtsi/Arf4Gt(AY0614)Wtsi (more )
Structure Level Pattern Image Note
TS11: parietal endoderm Weak 5B
TS11: extraembryonic visceral endoderm Strong 5B

Specimen 5C +/-: embryonic day 8.5; Arf4Gt(AY0614)Wtsi/Arf4+ (more )
Structure Level Pattern Image Note
TS13: allantois Present 5C +/-
TS13: paraxial mesenchyme Present 5C +/-
TS13: embryo endoderm Present Regionally restricted 5C +/- Expression was detected in the forming definitive endoderm in the hindgut region.
TS13: extraembryonic endoderm Present Regionally restricted 5C +/- Expression was detected in the visceral endoderm.

Specimen 5C -/-: embryonic day 8.5; Arf4Gt(AY0614)Wtsi/Arf4Gt(AY0614)Wtsi (more )
Structure Level Pattern Image Note
TS13: allantois Present 5C -/-
TS13: paraxial mesenchyme Present 5C -/-
TS13: embryo endoderm Present Regionally restricted 5C -/- Expression was detected in the forming definitive endoderm in the hindgut region.
TS13: extraembryonic endoderm Present Regionally restricted 5C -/- Expression was detected in the visceral endoderm.

Specimen 5D: embryonic day 8.5; Arf4Gt(AY0614)Wtsi/Arf4Gt(AY0614)Wtsi (more )
Structure Level Pattern Image Note
TS13: paraxial mesenchyme Present 5D
TS13: embryo endoderm Present Regionally restricted 5D Expression was detected in the forming definitive endoderm in the hindgut region.
TS13: extraembryonic endoderm Present Regionally restricted 5D Expression was detected in the visceral endoderm.

Specimen 5E +/-: embryonic day 9.5; Arf4Gt(AY0614)Wtsi/Arf4+ (more )
Structure Level Pattern Image Note
TS15: embryo Present 5E +/- conceptus , 5E +/- embryo
TS15: yolk sac Present 5E +/- yolk sac , 5E +/- conceptus

Specimen 5E -/-: embryonic day 9.5; Arf4Gt(AY0614)Wtsi/Arf4Gt(AY0614)Wtsi (more )
Structure Level Pattern Image Note
TS15: embryo Present 5E -/- embryo , 5E -/- conceptus
TS15: yolk sac Present 5E -/- yolk sac , 5E -/- conceptus

Specimen 5F top: embryonic day 9.5; Arf4Gt(AY0614)Wtsi/Arf4Gt(AY0614)Wtsi (more )
Structure Level Pattern Image Note
TS15: yolk sac endoderm Strong Regionally restricted 5F top Expression was strong in the visceral endoderm layer of the yolk sac.
TS15: hepatic primordium Strong Regionally restricted 5F top Expression was strong in the liver bud endoderm.

Specimen 5F middle: embryonic day 9.5; Arf4Gt(AY0614)Wtsi/Arf4Gt(AY0614)Wtsi (more )
Structure Level Pattern Image Note
TS15: yolk sac endoderm Strong Regionally restricted 5F middle Expression was strong in the visceral endoderm layer of the yolk sac.
TS15: gut endoderm Strong Regionally restricted 5F middle Expression was strong in the developing gut tube endoderm.

Specimen 5F bottom: embryonic day 9.5; Arf4Gt(AY0614)Wtsi/Arf4Gt(AY0614)Wtsi (more )
Structure Level Pattern Image Note
TS15: yolk sac endoderm Strong Regionally restricted 5F bottom Expression was strong in the visceral endoderm layer of the yolk sac.

Specimen S2A: embryonic day 7.0; Arf4Gt(AY0614)Wtsi/Arf4Gt(AY0614)Wtsi (more )
Structure Level Pattern Image Note
TS10: extraembryonic visceral endoderm Present S2A
TS10: parietal endoderm Present S2A

Specimen S2B: embryonic day 7.0; Arf4Gt(AY0614)Wtsi/Arf4Gt(AY0614)Wtsi (more )
Structure Level Pattern Image Note
TS10: extraembryonic visceral endoderm Present S2B
TS10: parietal endoderm Present S2B

Specimen S2C: embryonic day 7.0; Arf4Gt(AY0614)Wtsi/Arf4Gt(AY0614)Wtsi (more )
Structure Level Pattern Image Note
TS10: extraembryonic visceral endoderm Present S2C
TS10: parietal endoderm Present S2C

Specimen S2D: embryonic day 7.0; Arf4Gt(AY0614)Wtsi/Arf4Gt(AY0614)Wtsi (more )
Structure Level Pattern Image Note
TS10: primitive endoderm Present S2D

Specimen S3B: embryonic day 8.0; Arf4Gt(AY0614)Wtsi/Arf4Gt(AY0614)Wtsi (more )
Structure Level Pattern Image Note
TS12: allantois Present S3B
TS12: extraembryonic visceral endoderm Present S3B

Specimen S3C: embryonic day 8.0; Arf4Gt(AY0614)Wtsi/Arf4Gt(AY0614)Wtsi (more )
Structure Level Pattern Image Note
TS12: allantois Present S3C
TS12: extraembryonic visceral endoderm Present S3C

Specimen S3D: embryonic day 8.0; Arf4Gt(AY0614)Wtsi/Arf4Gt(AY0614)Wtsi (more )
Structure Level Pattern Image Note
TS12: paraxial mesenchyme Present S3D
TS12: primitive endoderm Present S3D

Specimen S3E: embryonic day 8.0; Arf4Gt(AY0614)Wtsi/Arf4Gt(AY0614)Wtsi (more )
Structure Level Pattern Image Note
TS12: paraxial mesenchyme Present S3E
TS12: primitive endoderm Present S3E


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory