Antibody:	anti-Lyve-1
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: renal cortex	Present	Regionally restricted	1a	Expression only in the cortex.
TS28: inner renal medulla	Absent		1a
TS28: outer renal medulla	Absent		1a
TS28: renal interstitium	Absent		1a
TS28: renal tubule	Absent		1a

Structure	Level	Pattern	Image	Note
TS28: renal lymphatic	Present	Regionally restricted	1a inset	Expression in lymphatic vessels around the arcuate artery.
TS28: arcuate artery	Absent		1a inset
TS28: arcuate vein	Absent		1a inset

Structure	Level	Pattern	Image	Note
TS28: renal lymphatic	Present	Regionally restricted	1b	Expression in lymphatic vessels distributed along the arcuate artery.
TS28: glomerular capillary system	Present	Regionally restricted	1b	Expression in endothelial cells in the glomerulus.

Structure	Level	Pattern	Image	Note
TS28: renal lymphatic	Present	Regionally restricted	1c	Expression in lymphatic vessels distributed along the interlobular artery.
TS28: glomerular capillary system	Present	Regionally restricted	1c	Expression in endothelial cells in the glomerulus.

Structure	Level	Pattern	Image	Note
TS28: renal lymphatic	Present	Regionally restricted	1d	Expression in lymphatic vessels distributed along the afferent arteriole.
TS28: glomerular capillary system	Present	Regionally restricted	1d	Expression in endothelial cells of the glomerular capillaries.

Structure	Level	Pattern	Image	Note
TS28: glomerular capillary system	Present	Regionally restricted	1e	Expression in endothelial cells in the glomerulus.

Structure	Level	Pattern	Image	Note
TS28: glomerular capillary system	Present	Regionally restricted	1f	Expression in endothelial cells in the glomerulus.

Structure	Level	Pattern	Image	Note
TS28: renal lymphatic	Present	Regionally restricted	1g	Expression in lymphatic vessels distributed along the interlobular artery.
TS28: glomerular capillary system	Present	Regionally restricted	1g	Expression in endothelial cells in the glomerulus.

Structure	Level	Pattern	Image	Note
TS28: glomerular capillary system	Present	Regionally restricted	1g'	Expression in glomerular endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: glomerular capillary system	Present	Regionally restricted	1g''	Expression in glomerular endothelial cells.
TS28: renal capillary	Present	Regionally restricted	1g''	Expression in extraglomerular lymphatic capillary.

Structure	Level	Pattern	Image	Note
TS28: glomerular capillary system	Present	Regionally restricted	1g'''	Expression in glomerular endothelial cells.
TS28: renal capillary	Present	Regionally restricted	1g'''	Expression in extraglomerular lymphatic capillary.

Structure	Level	Pattern	Image	Note
TS28: glomerular capillary system	Present	Regionally restricted	1g''''	Expression in glomerular endothelial cells and extraglomerular lymphatic capillary.
TS28: renal capillary	Present	Regionally restricted	1g''''	Expression in extraglomerular lymphatic capillary.

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Widespread	2a	Expression in large number of single cells distributed throughout the embryo.

Structure	Level	Pattern	Image	Note
TS20: lymphatic system	Strong	Regionally restricted	2b	Strong expression in well-developed extra-renal lymphatic vessels. No expression in lymphatic vessels in kidney.

Structure	Level	Pattern	Image	Note
TS20: lymphatic system	Present	Regionally restricted	2c' , 2c	Weak expression in lymphatic vessel extending to kidney. No expression in expression in lymphatic vessels in the kidney.

Structure	Level	Pattern	Image	Note
TS20: renal interstitium	Present	Single cells	2d	Expression in a few single cells in kidney, mainly in the medulla.

Structure	Level	Pattern	Image	Note
TS21: lymphatic system	Present	Regionally restricted	2e' , 2e'' , 2e	Expression in extra-renal lymphatics extending into the kidney and forming a rich plexus in the renal hilum.
TS21: renal interstitium	Present	Single cells	2e , 2e'' , 2e'	Expression in many single cells closely intermingled around the lymphatic plexus.

Structure	Level	Pattern	Image	Note
TS21: lymphatic system	Present	Regionally restricted	3a	Strong expression in extra-renal lymphatic vessels. Weak expression in lymphatic vessels extending from extra renal lymphatic vessels. No expression in lymphatic vessels in the medulla.
TS21: renal medulla	Present	Single cells	3a

Structure	Level	Pattern	Image	Note
TS22: lymphatic system	Present	Regionally restricted	3b	Increased expression in well-developed lymphatic plexuses. No expression in lymphatic vessels in the medulla.

Structure	Level	Pattern	Image	Note
TS22: lymphatic system	Present	Regionally restricted	3b'	Expression in filopodia-like sprouts of occuring on the end of growing lymphatic vessels. No expression in lymphatic vessels in the medulla.

Structure	Level	Pattern	Image	Note
TS24: renal cortical lymphatics	Present	Regionally restricted	3c	Expression in lymphatic vessels gradually forming along arcuate and interlobular blood vessels in the cortex.
TS24: renal medullary lymphatics	Absent		3c
TS24: renal medullary interstitium	Present	Single cells	3c	Expression in increasing numbers of single cells especially in the medulla.

Structure	Level	Pattern	Image	Note
TS28: renal cortical lymphatics	Present	Regionally restricted	3d	Expression in lymphatic vessels gradually forming along arcuate and interlobular blood vessels in the cortex.
TS28: renal medullary lymphatics	Absent		3d
TS28: renal medullary interstitium	Present	Single cells	3d	Expression in increasing numbers of single cells especially in the medulla.

Structure	Level	Pattern	Image	Note
TS28: renal papilla	Present	Single cells	3d'	Expression in single cells in the renal papilla.
TS28: renal medullary lymphatics	Absent		3d'

Structure	Level	Pattern	Image	Note
TS28: renal cortical lymphatics	Present	Regionally restricted	3e	Expression in lymphatic vessels gradually forming along arcuate and interlobular blood vessels in the cortex.
TS28: renal medullary lymphatics	Absent		3e
TS28: renal medullary interstitium	Present	Single cells	3e	Expression in single cells in the medulla medulla gradually decreased.

Structure	Level	Pattern	Image	Note
TS28: renal cortical lymphatics	Present	Regionally restricted	3f	Expression in lymphatic vessels gradually forming along arcuate and interlobular blood vessels in the cortex.
TS28: renal medullary lymphatics	Absent		3f
TS28: renal medullary interstitium	Absent		3f	No expression in single cells in the medulla.

Structure	Level	Pattern	Image	Note
TS28: renal cortical lymphatics	Present	Regionally restricted	3g	Expression in lymphatic vessels gradually forming along arcuate and interlobular blood vessels in the cortex.
TS28: renal medullary lymphatics	Absent		3g
TS28: renal medullary interstitium	Absent		3g	No expression in single cells in the medulla.

Structure	Level	Pattern	Image	Note
TS22: renal lymphatic	Present	Regionally restricted	4a	Expression in lymphatic vessels around arcuate veins.
TS22: renal vasculature	Present	Regionally restricted	4a	Expression in endothelial cells in branching buds of arcuate veins. No expression in endothelial cells in branching buds of arcuate arteries

Structure	Level	Pattern	Image	Note
TS22: renal lymphatic	Present	Regionally restricted	4b	Expression in lymphatic vessels around arcuate veins.
TS22: renal vasculature	Present	Regionally restricted	4b	Expression in endothelial cells in branching buds of arcuate veins. No expression in endothelial cells in branching buds of arcuate arteries.

Structure	Level	Pattern	Image	Note
TS22: renal lymphatic	Present	Regionally restricted	4c	Expression in lymphatic vessels around arcuate veins.
TS22: renal vasculature	Present	Regionally restricted	4c	Expression in endothelial cells in branching buds of arcuate veins. No expression in endothelial cells in branching buds of arcuate arteries.

Structure	Level	Pattern	Image	Note
TS26: renal lymphatic	Present	Regionally restricted	4d	Expression in lymphatic vessels around arcuate veins.
TS26: arcuate vein	Present	Regionally restricted	4d	Expression in endothelial cells in branching buds of arcuate veins.
TS26: arcuate artery	Absent		4d	No expression in endothelial cells in branching buds of arcuate arteries.

Structure	Level	Pattern	Image	Note
TS26: renal lymphatic	Present	Regionally restricted	4e	Expression in lymphatic vessels around arcuate veins.
TS26: arcuate vein	Present	Regionally restricted	4e	Expression in endothelial cells in branching buds of arcuate veins.
TS26: arcuate artery	Absent		4e	No expression in endothelial cells in branching buds of arcuate arteries.

Structure	Level	Pattern	Image	Note
TS26: renal lymphatic	Present	Regionally restricted	4f	Expression in lymphatic vessels around arcuate veins.
TS26: arcuate vein	Present	Regionally restricted	4f	Expression in endothelial cells in branching buds of arcuate veins.
TS26: arcuate artery	Absent		4f	No expression in endothelial cells in branching buds of arcuate arteries.

Structure	Level	Pattern	Image	Note
TS28: arcuate vein	Present	Regionally restricted	4g	Expression in endothelial cells in branching buds of arcuate veins.
TS28: arcuate artery	Absent		4g	No expression in endothelial cells in branching buds of arcuate arteries.

Structure	Level	Pattern	Image	Note
TS28: arcuate vein	Present	Regionally restricted	4h	Expression in endothelial cells in branching buds of arcuate veins.
TS28: arcuate artery	Absent		4h	No expression in endothelial cells in branching buds of arcuate arteries.

Structure	Level	Pattern	Image	Note
TS28: arcuate vein	Present	Regionally restricted	4i	Expression in endothelial cells in branching buds of arcuate veins.
TS28: arcuate artery	Absent		4i	No expression in endothelial cells in branching buds of arcuate arteries.

Structure	Level	Pattern	Image	Note
TS28: renal lymphatic	Present	Regionally restricted	4j	Expression in lymphatic vessels located around arcuate arteries and interlobular veins with thick smooth muscles.

Structure	Level	Pattern	Image	Note
TS28: renal lymphatic	Present	Regionally restricted	4k	Expression in lymphatic vessels located around arcuate arteries and interlobular veins with thick smooth muscles.

Structure	Level	Pattern	Image	Note
TS28: renal lymphatic	Present	Regionally restricted	4l	Expression in lymphatic vessels located around arcuate arteries and interlobular veins with thick smooth muscles.

Structure	Level	Pattern	Image	Note
TS21: renal interstitium	Present	Single cells	5a
TS21: s-shaped body	Absent		5a	No expression in early stage of developing nephron, including the vesicles of S-shaped body.

Structure	Level	Pattern	Image	Note
TS23: presumptive endothelium of capillary loop renal corpuscle	Present		5b	Expression in endothelial cells appearing first in the glomerulus of stage III nephrons.

Structure	Level	Pattern	Image	Note
TS24: maturing renal corpuscle	Present	Regionally restricted	5c	Expression in endothelial cells in the glomerulus of stage IV nephrons.

Structure	Level	Pattern	Image	Note
TS26: glomerular capillary system	Present	Regionally restricted	5d	Expression in endothelial cells in the glomerulus.
TS26: renal lymphatic	Present	Regionally restricted	5d	Expression in lymphatic capillary located adjacent to Bowman capsule.

Structure	Level	Pattern	Image	Note
TS28: renal vasculature	Present	Regionally restricted	5e	Expression in variable numbers of endothelial cells in the kidney.

Structure	Level	Pattern	Image	Note
TS28: renal vasculature	Present	Regionally restricted	5f	Expression in variable numbers of endothelial cells in the kidney.

Structure	Level	Pattern	Image	Note
TS21: renal interstitium	Present	Single cells	6a	Expression in single cells with thick irregular cytoplasmic processes.

Structure	Level	Pattern	Image	Note
TS23: renal interstitium	Present	Regionally restricted	6b	Expression in single cells with thick irregular cytoplasmic processes.

Structure	Level	Pattern	Image	Note
TS24: renal interstitium	Present	Single cells	6c	Expression in single cells with thick irregular cytoplasmic processes.

Structure	Level	Pattern	Image	Note
TS27: renal interstitium	Present	Single cells	6d	Expression in single cells with thick irregular cytoplasmic processes.

Structure	Level	Pattern	Image	Note
TS28: renal interstitium	Present	Single cells	6e	Expression in single cells with thick irregular cytoplasmic processes.

Structure	Level	Pattern	Image	Note
TS28: renal interstitium	Present	Single cells	6f	Expression in single cells with thick irregular cytoplasmic processes.

Structure	Level	Pattern	Image	Note
TS28: inner medullary interstitium	Present	Single cells	6f upper inset	Decreased expression in single cells located in deeper portion of inner medulla.

Structure	Level	Pattern	Image	Note
TS28: inner medullary interstitium	Present	Single cells	6f lower inset	Decreased expression in single cells located in deeper portion of inner medulla.