Gene Expression Data
Assay Details
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Assay
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Reference:
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J:84432
Qi Y, et al., Gli2 is required for normal Shh signaling and oligodendrocyte development in the spinal cord. Mol Cell Neurosci. 2003 Jul;23(3):440-50
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5694487
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh probe109
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1A:
embryonic day 10.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1G:
embryonic day 12.0
(more )
Specimen
1G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7A:
embryonic day 12.5
(more )
Specimen
7A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: medial ganglionic eminence |
Present |
Regionally restricted |
7B
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There was no difference in the expression in the medial ganglionic eminence in the mutant.
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| TS20: telencephalon |
Present |
Regionally restricted |
7B
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There was no difference in the expression in the anterior peduncular area in the mutant.
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| TS20: hypothalamus |
Present |
Regionally restricted |
7B
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Expression was detected in the midline in the mutant hypothalamus.
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Specimen
8A:
embryonic day 14.5
(more )
Specimen
8A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: forebrain |
Weak |
Regionally restricted |
8A
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Expression in the ventral forebrain was much downregulated at E14.5.
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| TS22: corpus striatum |
Weak |
Regionally restricted |
8A
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A weak expression was observed at the junction site of striatum and septum.
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| TS22: telencephalon septum |
Weak |
Regionally restricted |
8A
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A weak expression was observed at the junction site of striatum and septum.
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Specimen
8B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: corpus striatum |
Weak |
Regionally restricted |
8B
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A weak expression at the junction site of striatum and septum in the mutant was similar to the wild type.
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| TS22: telencephalon septum |
Weak |
Regionally restricted |
8B
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A weak expression at the junction site of striatum and septum in the mutant was similar to the wild type.
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Analysis Tools