Probe:	Netrin-1 cDNA
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS19: limb	Present		2F
TS19: embryo mesenchyme	Present	Widespread	2F	Expressed in the mesenchymal tissues distributed over the whole embryo.

Structure	Level	Pattern	Image	Note
TS20: optic stalk	Present	Regionally restricted	2M	Expressed in the optic nerve.

Structure	Level	Pattern	Image	Note
TS20: mammary bud	Present		2P lower

Structure	Level	Pattern	Image	Note
TS20: mammary bud	Present	Regionally restricted	2T	Mesenchymal expression, seen as an elliptical ring around the clear epithelial bud.

Structure	Level	Pattern	Image	Note
TS20: mammary bud	Present	Regionally restricted	2W	Expressed in the underlying loose mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud ectoderm	Present	Regionally restricted	3S	Expressed in the ectoderm at the base of the limb bud in the central distal ectoderm.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit epithelium	Present	Regionally restricted	3T	The distal expression expands to include the ectoderm overlying the digits, the nail primordial and peridigital stripes.
TS20: forelimb epithelium	Present	Regionally restricted	3T	The distal expression expands to include the ectoderm overlying the digits, the nail primordial and peridigital stripes.

Structure	Level	Pattern	Image	Note
TS20: hand epithelium	Present	Regionally restricted	3U	Expressed in the ectoderm overlying the digits, the nail primordial and peridigital stripes.

Structure	Level	Pattern	Image	Note
TS21: forelimb digit epithelium	Present	Regionally restricted	3V	The distal expression expands to include the ectoderm overlying the digits, the nail primordial and peridigital stripes.
TS21: forelimb epithelium	Present	Regionally restricted	3V	The distal expression expands to include the ectoderm overlying the digits, the nail primordial and peridigital stripes.

Structure	Level	Pattern	Image	Note
TS21: forelimb digit epithelium	Present	Regionally restricted	3W	Expressed in the ectoderm overlying the digits, the nail primordial and peridigital stripes.

Structure	Level	Pattern	Image	Note
TS22: hand joint	Present	Regionally restricted	3X	Expressed in anterior and posterior ectodermal spots at sites of joint formation.
TS22: hand skin	Present	Regionally restricted	3X	Expressed in anterior and posterior ectodermal spots at sites of joint formation.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit epithelium	Present	Regionally restricted	3e	Expressed in the surface ectoderm overlying the digits, but excluded from the interdigital ectoderm.

Structure	Level	Pattern	Image	Note
TS21: hair follicle	Present
TS21: vibrissa follicle	Present