Gene Expression Data
Assay Details
Assay
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Reference:
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J:187950
Western P, et al., Analysis of Esg1 expression in pluripotent cells and the germline reveals similarities with Oct4 and Sox2 and differences between human pluripotent cell lines. Stem Cells. 2005 Nov-Dec;23(10):1436-42
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5693915
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Gene symbol:
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Dppa5a
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Gene name:
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developmental pluripotency associated 5A
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Antibody:
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anti-ESG1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 594
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Results
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Specimen
2A Zyg:
embryonic day 0.5
(more )
Note: Zygote. Double labeled: red - Dppa5a; green - Pou5f1 (assay MGI:5693916).
Specimen
2A Zyg:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 0.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Zygote. Double labeled: red - Dppa5a; green - Pou5f1 (assay MGI:5693916).
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Structure |
Level |
Pattern |
Image |
Note |
TS1: 1-cell stage embryo |
Present |
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2A Zyg ESG1
,
2A Zyg Merge
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Expression is in the cytoplasm.
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Specimen
2A 8 cell:
embryonic day 2.5
(more )
Note: 8 cell embryo. Double labeled: red - Dppa5a; green - Pou5f1 (assay MGI:5693916).
Specimen
2A 8 cell:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 2.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8 cell embryo. Double labeled: red - Dppa5a; green - Pou5f1 (assay MGI:5693916).
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Structure |
Level |
Pattern |
Image |
Note |
TS3: 8-cell stage embryo |
Present |
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2A 8 cell Merge
,
2A 8 cell ESG1
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Expression is in the cytoplasm.
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Specimen
2A E3.5:
embryonic day 3.5
(more )
Note: Double labeled: red - Dppa5a; green - Pou5f1 (assay MGI:5693916).
Specimen
2A E3.5:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Dppa5a; green - Pou5f1 (assay MGI:5693916).
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: inner cell mass |
Present |
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2A E3.5 Merge
,
2A E3.5 ESG1
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Expression is in the cytoplasm.
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Specimen
2A E4.5:
embryonic day 4.5
(more )
Note: Double labeled: red - Dppa5a; green - Pou5f1 (assay MGI:5693916).
Specimen
2A E4.5:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 4.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Dppa5a; green - Pou5f1 (assay MGI:5693916).
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Structure |
Level |
Pattern |
Image |
Note |
TS6: embryo |
Present |
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2A E4.4 ESG1
,
2A E4.5 Merge
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Expression is in the cytoplasm and nucleus.
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TS6: trophectoderm |
Present |
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2A E4.4 ESG1
,
2A E4.5 Merge
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Expression is in the cytoplasm and nucleus.
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