Gene Expression Data
Assay Details
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Assay
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Reference:
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J:117269
Lavado A, et al., Prox1 expression patterns in the developing and adult murine brain. Dev Dyn. 2007 Feb;236(2):518-24
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5693811
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Gene symbol:
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Prox1
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Gene name:
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prospero homeobox 1
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Antibody:
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anti-NH-Prox1
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Detection system:
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Secondary antibody coupled to Cy3
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Assay notes:
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Similar results were obtained using two antibodies; it is possible MGI:5555854 was used to obtain some or all of the pictured data.
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Results
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Specimen
2A:
embryonic day 12.5
(more  )
Note: Double labeled: red - Prox1; green - Tubb3 (assay MGI:5693855).
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Prox1; green - Tubb3 (assay MGI:5693855).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: ganglionic eminence |
Present |
Regionally restricted |
2A
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Expression is primarily present in the subventricular zone; a few expressing cells are found in the mantle zone. Not all cells co-express the neuronal marker.
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Specimen
2B:
embryonic day 12.5
(more )
Note: Dividing cells were labeled with an antibody to phosphorylated histone; these cells are green.
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Dividing cells were labeled with an antibody to phosphorylated histone; these cells are green.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: ganglionic eminence |
Present |
Regionally restricted |
2B
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Expression is primarily present in post-mitotic cells. Most expression is found in the subventricular zone; a few expressing cells are found in the mantle zone.
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Specimen
2C:
embryonic day 14.5
(more )
Note: Double labeled: red - Prox1; green - Tubb3 (assay MGI:5693855).
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Prox1; green - Tubb3 (assay MGI:5693855).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: ganglionic eminence |
Present |
Regionally restricted |
2C
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Expression is primarily present in the subventricular zone; a few expressing cells are found in the mantle zone. The inset is a higher magnification, illustrating examples of double labeling. However, not all cells are double labeled.
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Specimen
2D:
embryonic day 14.5
(more )
Note: Dividing cells were labeled with an antibody to phosphorylated histone; these cells are green.
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Dividing cells were labeled with an antibody to phosphorylated histone; these cells are green.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: ganglionic eminence |
Present |
Regionally restricted |
2D
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Expression is primarily present post-mitotic cells. Expression is primarily present in the subventricular zone; a few expressing cells are found in the mantle zone.
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Specimen
2E:
embryonic day 14.5
(more )
Note: Double labeled: red - Prox1; green - Tubb3 (assay MGI:5693855).
Specimen
2E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Prox1; green - Tubb3 (assay MGI:5693855).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hypothalamus |
Present |
Regionally restricted |
2E
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Expression is present in the preoptic area.
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Specimen
2F:
embryonic day 14.5
(more )
Note: Dividing cells were labeled with an antibody to phosphorylated histone; these cells are green.
Specimen
2F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Dividing cells were labeled with an antibody to phosphorylated histone; these cells are green.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hypothalamus |
Present |
Regionally restricted |
2F
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Expression is primarily present post-mitotic cells, in this case in the preoptic area.
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Specimen
2G:
embryonic day 14.5
(more )
Note: Double labeled: red - Prox1; green - Tubb3 (assay MGI:5693855).
Specimen
2G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Prox1; green - Tubb3 (assay MGI:5693855).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hippocampus |
Present |
Regionally restricted |
2G
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Expression is present in dentate gyrus neuroepithelium.
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Specimen
2H:
embryonic day 14.5
(more )
Note: Dividing cells were labeled with an antibody to phosphorylated histone; these cells are green.
Specimen
2H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Dividing cells were labeled with an antibody to phosphorylated histone; these cells are green.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hippocampus |
Present |
Regionally restricted |
2H
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Expression is present in dentate gyrus neuroepithelium. Unlike in other areas of the brain, expressing cells are often still dividing.
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