Probe:	Six3 probe16
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: future brain	Present	Regionally restricted		Expression is present in the anterior neural ectoderm.

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon neural fold	Strong		2F	Expression in the anterior neural ridge and anterior neuroectoderm.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	2G	Expression in the ventral forebrain.
TS13: eye	Present		2G	Expression in the optic vesicle.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	2H	Expression in the ventral forebrain.
TS15: eye	Present		2H	Expression in the optic vesicle.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	2I	Expression in the ventral forebrain.
TS17: eye	Present		2I	Expression in the optic vesicle.

Structure	Level	Pattern	Image	Note
TS22: ganglionic eminence	Present		3B
TS22: tectum	Present		3B
TS22: thalamus	Present	Regionally restricted	3B	Expression in the ventral thalamus.

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Present	Regionally restricted	3D	Expression restricted to the ventricular zone.
TS22: hypothalamus mantle layer	Present		3D
TS22: hypothalamus ventricular layer	Present		3D
TS22: thalamus	Present	Regionally restricted	3D	Expression in the ventral thalamus.
TS22: midbrain tegmentum	Present		3D
TS22: pituitary gland	Present		3D

Structure	Level	Pattern	Image	Note
TS26: midbrain tegmentum	Present		3F
TS26: tectum	Present		3F
TS26: telencephalon septum	Present		3F
TS26: thalamus	Present	Regionally restricted	3F	Expression in the ventral thalamus.
TS26: pituitary gland	Present		3F

Structure	Level	Pattern	Image	Note
TS26: midbrain tegmentum	Present		3H
TS26: ganglionic eminence	Present		3H
TS26: thalamus	Present	Regionally restricted	3H	Expression in the ventral thalamus.
TS26: hypothalamus	Present		3H
TS26: pituitary gland	Present		3H