Antibody:	Anti-Rgmb (AF3597)
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Strong	Regionally restricted	1E	Strong staining was noted on olfactory sensory neuron axons.
TS24: main olfactory bulb external plexiform layer	Present	Regionally restricted	1E	Expression was detected in mitral cell axons in the internal and external plexiform layers.
TS24: main olfactory bulb internal plexiform layer	Present	Regionally restricted	1E	Expression was detected in mitral cell axons in the internal and external plexiform layers.
TS24: main olfactory bulb prospective glomerular layer	Present		1E
TS24: vomeronasal organ	Present		1E

Structure	Level	Pattern	Image	Note
TS24: olfactory tract	Present	Regionally restricted	1I	Expression was detected in axons of the lateral olfactory tract.
TS24: diencephalon	Weak	Regionally restricted	1I	Expression was detected in axons of the anterior commissure.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Weak	Regionally restricted	3E , 3E'	Expression was on axons.
TS24: corpus callosum	Strong		3E

Structure	Level	Pattern	Image	Note
TS24: cerebral internal capsule	Weak	Regionally restricted	3H	Expression was on axons.
TS24: corpus striatum	Weak	Regionally restricted	3H	Expression was on axons.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Trace		4E	Expression was virtually absent in the P5 cortex.

Structure	Level	Pattern	Image	Note
TS28: cerebral internal capsule	Present
TS28: corpus callosum	Present

Structure	Level	Pattern	Image	Note
TS28: corticospinal tract	Ambiguous		4H

Structure	Level	Pattern	Image	Note
TS24: telencephalon pia mater	Strong	Regionally restricted	6E	Expression was detected in the hippocampal pia.
TS24: hippocampus	Weak	Regionally restricted	6E	Weak expression was detected in the CA region and fimbria.
TS24: dentate gyrus primordium	Weak		6E

Structure	Level	Pattern	Image	Note
TS28: fimbria hippocampus	Weak		6N
TS28: hippocampus stratum lacunosum	Weak		6N
TS28: dentate gyrus molecular layer	Weak	Regionally restricted	6N	Weak expression was detected in the outer molecular layer of the dentate gyrus.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Absent
TS28: hippocampus stratum lacunosum	Weak
TS28: fimbria hippocampus	Present			Expression was reduced as compared to P5.

Structure	Level	Pattern	Image	Note
TS24: lateral habenular nucleus	Present		8H
TS24: medial habenular nucleus	Present		8H
TS24: stria medullaris	Present		8H

Structure	Level	Pattern	Image	Note
TS24: fasciculus retroflexus	Strong		8K

Structure	Level	Pattern	Image	Note
TS28: fasciculus retroflexus	Strong		9E

Structure	Level	Pattern	Image	Note
TS24: deep cerebellar nucleus	Present		11E
TS24: cerebellum external granule cell layer	Present	Regionally restricted	11E	Expression was confined to the inner part of the EGL.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		11K
TS28: cerebellum external granule cell layer	Present		11K
TS28: cerebellum internal granule cell layer	Present		11K

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		11Q
TS28: cerebellum granule cell layer	Present		11Q
TS28: cerebellum molecular layer	Present	Regionally restricted	11Q	Staining may represent the dendritic processes of Purkinje cells.