Probe:	Cyp26b1 cDNA2
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present		1C
TS20: vertebral pre-cartilage condensation	Present		1C
TS20: otic capsule	Moderate		1C
TS20: nasal process	Present	Regionally restricted	1C	There is increased expression toward the extremities.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present		1F
TS22: pons	Present		1F
TS22: spinal cord	Present		1F
TS22: vertebral pre-cartilage condensation	Present	Graded	1F	There is a caudal to cranial decreasing gradient of expression.
TS22: liver	Absent		1F

Structure	Level	Pattern	Image	Note
TS22: genital tubercle mesenchyme	Present		1F inset

Structure	Level	Pattern	Image	Note
TS20: forelimb pre-cartilage condensation	Present		2C
TS20: hand mesenchyme	Present	Regionally restricted	2C	Expression is in the anterior and posterior mesenchyme.
TS20: forelimb digit mesenchyme	Present	Regionally restricted	2C	Expression is in the very distal digit mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: limb digit	Present	Regionally restricted	2F	Expression is in tendons.
TS22: digit mesenchyme	Present	Regionally restricted	2F	Expression is in the very distal digit mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: arm	Present	Regionally restricted	2I	Expression is in the perichondrial areas and in tendons.
TS22: hair follicle	Present		2I
TS22: forelimb musculature	Absent		2I

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Present	Regionally restricted	2L	Expression is in the perichondrial areas.

Structure	Level	Pattern	Image	Note
TS22: pelvic girdle skeleton	Present	Regionally restricted	2O	Expression is in the perichondrial areas and in tendons.

Structure	Level	Pattern	Image	Note
TS22: corpus striatum	Present		3C
TS22: hair follicle	Present		3C
TS22: pigmented retinal epithelium	Present		3C
TS22: eyelid	Present	Regionally restricted	3C	Expression is in the mesenchyme underneath the palpebral fissure.

Structure	Level	Pattern	Image	Note
TS22: nose	Present	Regionally restricted	3D/E	Expression is in the recesses separating the nasal turbinates.

Structure	Level	Pattern	Image	Note
TS22: laryngeal cartilage	Present		3F/G
TS22: esophagus	Present	Regionally restricted	3F/G	Expression is at the level of the oesophageal-laryngeal bifurcation.
TS22: larynx	Present	Regionally restricted	3F/G	Expression is at the level of the oesophageal-laryngeal bifurcation.

Structure	Level	Pattern	Image	Note
TS22: mandible	Present		3J
TS22: palatal shelf	Present		3J
TS22: tongue mesenchyme	Present	Regionally restricted	3J	Expression is in the superficial tongue mesenchyme.
TS22: nose	Present	Regionally restricted	3J	There is ijcreased expression toward the extremities.

Structure	Level	Pattern	Image	Note
TS26: molar	Present		3M

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present		4A/B
TS22: hippocampus	Present		4A/B
TS22: pons	Present		4A/B
TS22: hindbrain	Present		4A/B	The anterior expression boundary is at the level of the isthmus.
TS22: midbrain-hindbrain junction	Present		4A/B	The anterior expression boundary is at the level of the isthmus.
TS22: tectum	Present	Regionally restricted	4A/B
TS22: hindbrain ventricular layer	Strong		4A/B	Strongest expression is in the inner layer.

Structure	Level	Pattern	Image	Note
TS24: corpus striatum	Present		4C/D

Structure	Level	Pattern	Image	Note
TS28: amygdala	Present		4E/F
TS28: hippocampus CA3	Present		4E/F
TS28: subiculum	Present		4E/F

Structure	Level	Pattern	Image	Note
TS28: amygdala	Present		4G/H
TS28: hippocampus CA3	Present		4G/H
TS28: subiculum	Present		4G/H
TS28: meninges	Present	Regionally restricted	4G/H	Expression is in the inner meningeal layer.

Structure	Level	Pattern	Image	Note
TS28: pia mater	Present		4H inset

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Regionally restricted	5B	Expression is in the upper and lower thoracic regions.

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Regionally restricted	5C	Expression is in the upper and lower thoracic regions.

Structure	Level	Pattern	Image	Note
TS24: spinal cord lateral wall	Present	Regionally restricted	5D	Expression is in the upper and lower thoracic regions.

Structure	Level	Pattern	Image	Note
TS24: spinal cord lateral wall	Present	Regionally restricted	5E	Expression is in the upper and lower thoracic regions.

Structure	Level	Pattern	Image	Note
TS24: spinal cord lateral wall	Present	Regionally restricted	5F	Expression is in the upper and lower thoracic regions.
TS24: lung	Weak		5F

Structure	Level	Pattern	Image	Note
TS22: outflow tract	Present	Regionally restricted	6C	Expression is in endocardial cells.

Structure	Level	Pattern	Image	Note
TS26: lung	Weak		6F

Structure	Level	Pattern	Image	Note
TS22: spleen primordium	Present		6I
TS22: liver	Absent		6I

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present	Punctate	6L

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Present	Regionally restricted	7A/B	Expression is in dermal cells surrounding the epithelium.

Structure	Level	Pattern	Image	Note
TS26: dermis	Present	Widespread	7C/D

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Present

Structure	Level	Pattern	Image	Note
TS22: outer ear	Present	Regionally restricted		Expression is in the mesenchyme.
TS22: thymus primordium	Present