Gene Expression Data
Assay Details
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Assay
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Reference:
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J:117178
Schmidl M, et al., Twisted gastrulation modulates bone morphogenetic protein-induced collagen II and X expression in chondrocytes in vitro and in vivo. J Biol Chem. 2006 Oct 20;281(42):31790-800
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5691950
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Gene symbol:
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Twsg1
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Gene name:
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twisted gastrulation BMP signaling modulator 1
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Probe:
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Tsg cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The authors state that identical results were obtained with this probe and a probe of the 3' untranslated region: Tsg cDNA2 MGI:5691794. These are data not shown.
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Results
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Specimen
E15.5:
embryonic day 15.5
(more  )
Specimen
E15.5:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: humerus |
Strong |
Regionally restricted |
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Expression is in the hypertrophic zone.
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| TS23: rib |
Present |
Regionally restricted |
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Expression is in the hypertrophic zone.
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| TS23: turbinate bone primordium |
Present |
Regionally restricted |
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Expression is in cartilage primordia.
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| TS23: vertebra |
Present |
Regionally restricted |
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Expression is in cartilage.
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Specimen
E17.5:
embryonic day 17.5
(more )
Specimen
E17.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: long bone |
Present |
Regionally restricted |
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Expression is in the hypertrophic zone of the growth plates and is weak in the proliferating and resting cartilage and is absent in the proliferating zone of the epiphysis. Expression is in endochondral and periosteal trabeculae of long bones.
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| TS25: humerus |
Present |
Regionally restricted |
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Expression is in the hypertrophic zone of the growth plates and is weak in the proliferating and resting cartilage.
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| TS25: radius |
Present |
Regionally restricted |
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Expression is in the hypertrophic zone of the growth plates and is weak in the proliferating and resting cartilage.
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| TS25: digit mesenchyme |
Present |
Regionally restricted |
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Expression is in growth cartilage.
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| TS25: ulna |
Present |
Regionally restricted |
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Expression is in the hypertrophic zone of the growth plates and is weak in the proliferating and resting cartilage.
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| TS25: metacarpus |
Present |
Regionally restricted |
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Expression is on the surface zone of a metacarpal joint.
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| TS25: scapula |
Present |
Regionally restricted |
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Expression is in the hypertrophic cartilage zone.
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| TS25: tibia |
Present |
Regionally restricted |
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Expression is in the hypertrophic cartilage zone.
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| TS25: hindlimb musculature |
Absent |
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| TS25: hindlimb skin |
Absent |
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Specimen
Newborn:
postnatal newborn
(more )
Specimen
Newborn:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: radius |
Present |
Regionally restricted |
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Expression is in the hypertrophic zone and is weak in the resting zone.
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| TS27: humerus |
Present |
Regionally restricted |
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Expression is in the hypertrophic cartilage zone.
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| TS27: scapula |
Present |
Regionally restricted |
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Expression is in the hypertrophic cartilage zone.
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| TS27: tibia |
Present |
Regionally restricted |
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Expression is in the hypertrophic cartilage zone.
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Specimen
P30:
postnatal day 30
(more )
Specimen
P30:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 30
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools