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Gene Expression Data
Assay Details
Assay
Reference: J:49266 Robb L, et al., epicardin: A novel basic helix-loop-helix transcription factor gene expressed in epicardium, branchial arch myoblasts, and mesenchyme of developing lung, gut, kidney, and gonads. Dev Dyn. 1998 Sep;213(1):105-13
Assay type: RNA in situ
MGI Accession ID: MGI:5691937
Gene symbol: Tcf21
Gene name: transcription factor 21
Probe: epicardin cDNA1
Probe preparation: Antisense labelled with P33 RNA
Visualized with: Autoradiography
Assay notes: The authors used two probes but did not specify which was used to generate these data; both gave similar results. It is possible MGI:5691931 was used instead.
Results
Specimen 6A/B: embryonic day 14.5 (more )
Note: sagittal section
Structure Level Pattern Image Note
TS22: visceral pericardium Present 6A/B Expression in epicardial layer of the heart.
TS22: lung parenchyma Present 6A/B

Specimen 6D/E: embryonic day 12.5 (more )
Note: sagittal section
Structure Level Pattern Image Note
TS20: midgut mesenchyme Present 6D/E

Specimen 6F: embryonic day 14.5 (more )
Note: sagittal section
Structure Level Pattern Image Note
TS22: esophagus mesenchyme Present 6F Expression is present in the submucosa and circular and longitudinal muscular layers.
TS22: large intestine mesenchyme Present 6F Expression is present in the submucosa and circular and longitudinal muscular layers.
TS22: rectum mesenchyme Present 6F Expression is present in the submucosa and circular and longitudinal muscular layers.
TS22: small intestine mesenchyme Present 6F Expression is present in the submucosa and circular and longitudinal muscular layers.
TS22: stomach mesenchyme Present 6F Expression is present in the submucosa and circular and longitudinal muscular layers.

Specimen 6G/H: embryonic day 12.5 (more )
Note: sagittal section
Structure Level Pattern Image Note
TS20: lung mesenchyme Present 6G/H
TS20: trachea mesenchyme Present 6G/H Expression in mesenchyme associated with the trachea.
TS20: main bronchus mesenchyme Present 6G/H Expression is mesenchyme surrounding the bronchi.

Specimen 6I: embryonic day 14.5 (more )
Note: sagittal section
Structure Level Pattern Image Note
TS22: lung parenchyma Present 6I
TS22: bronchus mesenchyme Present 6I Expression is present in non-epithelial components of the bronchi.

Specimen 6J: embryonic day 14.5 (more )
Note: sagittal section
Structure Level Pattern Image Note
TS22: stomach mesenchyme Present 6J
TS22: interstitium of the testis Strong 6J Expression is in the stroma.
TS22: primary sex cord Absent 6J
TS22: testis Present Regionally restricted 6J Expression is present in the epididymis and ductus deferens
TS22: metanephros Present 6J

Specimen 6K/L: embryonic day 14.5 (more )
Note: sagittal section
Structure Level Pattern Image Note
TS22: renal medullary interstitium Present 6K/L Expression is prominent in the stroma surrounding the collecting ducts.
TS22: maturing nephron Present Regionally restricted 6K/L Expression is present in the epithelial caps (podocytes) of well-differentiated metanephrons.

Specimen not shown: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: cap mesenchyme Present Expression is present in condensing mesenchyme surrounding the nephric tubule.

Specimen not shown: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: bronchus mesenchyme Present Expression is present in non-epithelial components of the bronchi.
TS26: lung parenchyma Present
TS26: spleen Present Expression is present in mesenchymal tissue.
TS26: adrenal cortex Present

Specimen not shown: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: ductus deferens Present
TS26: epididymis Present
TS26: testis Present

Specimen not shown: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: ovary Present
TS26: oviduct Present
TS26: uterine horn Present


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory