Probe:	epicardin cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	The authors used two probes but did not specify which was used to generate these data; both gave similar results. It is possible MGI:5691931 was used instead.

Structure	Level	Pattern	Image	Note
TS12: 1st branchial arch	Present	Regionally restricted		Expression in a small cluster of cells.

Structure	Level	Pattern	Image	Note
TS13: branchial arch	Present		5A	Expression encompasses the entire branchial region.
TS13: lateral plate mesoderm	Weak	Regionally restricted	5A	Expression is seen in caudal lateral plate (visceral) mesoderm.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mesenchyme derived from head mesoderm	Present	Regionally restricted	5B	Expression is present in the myoblasts.
TS15: 2nd branchial arch mesenchyme derived from head mesoderm	Present	Regionally restricted	5B	Expression is present in the myoblasts.
TS15: 3rd branchial arch mesenchyme derived from head mesoderm	Present	Regionally restricted	5B	Expression is present in the myoblasts.
TS15: proepicardium	Strong	Regionally restricted	5B	Expression is found in the septum transverum, in the region caudal to the heart.
TS15: visceral pericardium	Present	Clusters	5B	Expression is found in epicardial cells on the surface of the heart.
TS15: endocardial cushion	Present		5B	Expression in the endocardial cushion tissue of the atrioventricular canal and outflow tract.
TS15: mesenchyme derived from splanchnopleure	Weak		5B	Expression is found the caudal lateral plate (visceral) mesoderm.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mesenchyme derived from head mesoderm	Strong	Regionally restricted	5C	Expression in the myoblasts.
TS15: 2nd branchial arch mesenchyme derived from head mesoderm	Strong	Regionally restricted	5C	Expression in the myoblasts.
TS15: 3rd branchial arch mesenchyme derived from head mesoderm	Strong	Regionally restricted	5C	Expression in the myoblasts.
TS15: proepicardium	Strong		5C
TS15: visceral pericardium	Present	Clusters	5C	Expression is found in epicardial cells on the surface of the heart.
TS15: endocardial cushion	Present		5C	Expression is found in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch	Present		5D

Structure	Level	Pattern	Image	Note
TS15: proepicardium	Present		5E
TS15: visceral pericardium	Present	Clusters	5E	Expression is present in cells on the surface of the atrium and ventricle.

Structure	Level	Pattern	Image	Note
TS15: proepicardium	Present		5F
TS15: visceral pericardium	Present		5F

Structure	Level	Pattern	Image	Note
TS15: septum transversum	Present	Regionally restricted	5G	Expression is present in the proepicardium mesenchyme. No expression is seen in other mesenchymes, such that at the confluence of pericardium, amnion and yolk sac.
TS15: proepicardium	Present	Graded	5G

Structure	Level	Pattern	Image	Note
TS15: endocardial cushion	Present		5H	Expression in cushions of the atrioventricular canal.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mesenchyme derived from head mesoderm	Present	Regionally restricted	5I	Expression in myoblasts.

Structure	Level	Pattern	Image	Note
TS17: visceral pericardium	Present			Expression in the epicardium.
TS17: gut mesenchyme	Present			Expression in the caudal visceral mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: urogenital ridge	Weak			Expression is present in intermediate mesodermal derivatives.

Structure	Level	Pattern	Image	Note
TS22: heart valve	Present

Structure	Level	Pattern	Image	Note
TS23: gonad	Strong			Expression is present in the mesenchymal tissues.
TS23: paramesonephric duct	Present	Widespread		Expression is present in the mesenchymal tissues of all Mullerian duct derivatives.
TS23: nephric duct	Present	Widespread		Expression is present in the mesenchymal tissues of all Wolffian duct derivatives.

Structure	Level	Pattern	Image	Note
TS26: gut mesenchyme	Present			Expression in the caudal visceral mesenchyme.
TS26: visceral pericardium	Present			Expression in the epicardium.
TS26: spleen	Present