Probe:	Pbx4 probe
Probe preparation:	Antisense RNA

Structure	Cell Type	Level	Pattern	Image	Note
TS28: seminiferous tubule epithelium		Present	Regionally restricted	4B	Expression is at the luminal-most side of the epithelium.
TS28: primary spermatocyte		Present		4B	Pachytene spermatocytes. Absent in spermatocytes in the earliest stages of the meiotic prophase.
TS28: spermatogonium		Absent		4B
TS28: Sertoli cell	Sertoli cell	Absent		4B

Structure	Cell Type	Level	Pattern	Image	Note
TS28: seminiferous tubule epithelium		Present	Regionally restricted	4D	Expression is at the luminal-most side of the epithelium.
TS28: primary spermatocyte		Present		4D	Pachytene spermatocytes. Absent in spermatocytes in the earliest stages of the meiotic prophase.
TS28: spermatogonium		Absent		4D
TS28: Sertoli cell	Sertoli cell	Absent		4D

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule epithelium	Present		4F	Expression is in the spermatocyte-containing layers of the seminiferous epithelium and is absent in cells located towards the lumen of the tubules.
TS28: spermatid	Present		4F
TS28: spermatogonium	Absent		4F

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule epithelium	Present	Regionally restricted	4H	Expression is in the spermatocyte-containing layers of the seminiferous epithelium and is absent in cells located towards the lumen of the tubules.
TS28: spermatid	Present		4H
TS28: spermatogonium	Absent		4H

Structure	Level	Pattern	Image	Note
TS10: embryo	Absent			The authors state that no specific signal was visible in mouse embryos between E7.0 and E10.5.

Structure	Level	Pattern	Image	Note
TS17: embryo	Absent			The authors state that no specific signal was visible in mouse embryos between E7.0 and E10.5.