Probe:	Kcne1 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: heart	Absent

Structure	Level	Pattern	Image	Note
TS15: atrium cardiac muscle	Present	Widespread	4A	First expression at E9.5 throughout the entire myocardial tube. Atrial and ventricular myocardial champers display similar expression levels.
TS15: primitive ventricle cardiac muscle	Present	Widespread	4A	First expression at E9.5 throughout the entire myocardial tube. Atrial and ventricular myocardial champers display similar expression levels.
TS15: outflow tract	Strong	Regionally restricted	4A	High levels of expression in the distal most region of the outflow tract, at the boundary between outflow tract myocardium and the mesenchymal aortic sac.

Structure	Level	Pattern	Image	Note
TS15: atrium cardiac muscle	Present	Widespread	4B	First expression at E9.5 throughout the entire myocardial tube. Atrial and ventricular myocardial champers display similar expression levels.
TS15: primitive ventricle cardiac muscle	Present	Widespread	4B	First expression at E9.5 throughout the entire myocardial tube. Atrial and ventricular myocardial champers display similar expression levels.
TS15: outflow tract	Strong	Regionally restricted	4B	High levels of expression in the distal most region of the outflow tract, at the boundary between outflow tract myocardium and the mesenchymal aortic sac.

Structure	Level	Pattern	Image	Note
TS17: heart	Present			Authors state that expression profile is maintained at E10.5.

Structure	Level	Pattern	Image	Note
TS20: outflow tract cardiac muscle	Present		4C
TS20: ventricle cardiac muscle	Present		4C
TS20: atrioventricular canal	Absent		4C
TS20: atrium cardiac muscle	Absent		4C

Structure	Level	Pattern	Image	Note
TS20: outflow tract cardiac muscle	Present		4D
TS20: ventricle cardiac muscle	Present		4D
TS20: atrioventricular canal	Absent		4D
TS20: atrium cardiac muscle	Absent		4D

Structure	Level	Pattern	Image	Note
TS20: ventricle cardiac muscle	Present		4E
TS20: interventricular septum cardiac muscle	Strong		4E	Very high expression along the entire ventricular septum.

Structure	Level	Pattern	Image	Note
TS20: interventricular septum cardiac muscle	Strong		4F	Very high expression along the entire ventricular septum.
TS20: ventricle cardiac muscle	Present	Regionally restricted	4F	Ventricular trabeculations almost devoid of expression.

Structure	Level	Pattern	Image	Note
TS20: interventricular groove	Present	Regionally restricted		Enhanced expression in the myocardium forming the interventricular sulcus (groove) at both dorsal and ventral side.

Structure	Level	Pattern	Image	Note
TS21: heart	Present	Regionally restricted		Expression profile maintained at E13.5.

Structure	Level	Pattern	Image	Note
TS22: ventricle cardiac muscle	Present	Regionally restricted
TS22: interventricular septum	Absent

Structure	Level	Pattern	Image	Note
TS23: ventricle cardiac muscle	Present	Regionally restricted		Authors report that expression profile is sustained.
TS23: interventricular septum	Absent			Authors report that expression profile is sustained.

Structure	Level	Pattern	Image	Note
TS24: ventricle cardiac muscle	Present	Regionally restricted	4G	Expression mainly confined to outer compact myocardial fibers.
TS24: interventricular septum	Absent		4G

Structure	Level	Pattern	Image	Note
TS24: ventricle cardiac muscle	Present	Regionally restricted	4H	Expression mainly confined to outer compact myocardial fibers.
TS24: interventricular septum	Absent		4H

Structure	Level	Pattern	Image	Note
TS26: ventricle cardiac muscle	Present	Regionally restricted		Authors report that expression profile is sustained.
TS26: interventricular septum	Absent			Authors report that expression profile is sustained.