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Gene Expression Data
Assay Details
Assay
Reference: J:50943 Cho EA, et al., TCF-4 binds beta-catenin and is expressed in distinct regions of the embryonic brain and limbs. Mech Dev. 1998 Sep;77(1):9-18
Assay type: RNA in situ
MGI Accession ID: MGI:5689859
Gene symbol: Tcf7l2
Gene name: transcription factor 7 like 2, T cell specific, HMG box
Probe: mTcf-4 probe2
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Embryos were post-fixed in 0.2% glutaraldehyde/4% paraformaldehyde.
Results
Specimen 4A: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: embryo endoderm Present Regionally restricted 4A Expression is at the anterior endoderm.

Specimen 4B: embryonic day 7.0 (more )
Structure Level Pattern Image Note
TS10: embryo endoderm Weak Regionally restricted 4B Expression is at the most anterior endoderm.

Specimen 4C: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: diencephalon Strong 4C

Specimen 4E: embryonic day 8.5 (more )
Note: Bisected embryo
Structure Level Pattern Image Note
TS13: rhombomere 5 Strong 4E
TS13: rhombomere 1 Present 4E
TS13: rhombomere 3 Present 4E
TS13: future spinal cord neural plate Present Regionally restricted 4E Expression is at the most rostral border of the neural plate.

Specimen 4F: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: midbrain marginal layer Present 4F
TS21: thalamus Present 4F

Specimen 4G: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: diencephalon Strong 4G
TS19: fronto-nasal process Present 4G
TS19: limb Present 4G
TS19: midbrain Present Scattered 4G Expression is in a small number of scattered cells in the anteriormost part and lateral sides.

Specimen 4H: embryonic day 11.5; Pax6Sey/Pax6Sey (more )
Structure Level Pattern Image Note
TS19: fronto-nasal process Present 4H Expression in these tissues is the same as in wild-type embryos.
TS19: limb Present 4H Expression in these tissues is the same as in wild-type embryos.
TS19: diencephalon Absent 4H

Specimen 5A: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: forelimb bud Absent 5A

Specimen 5B: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: forelimb bud Present Regionally restricted 5B Expression is at the distal end and the proximal region.
TS19: hindlimb bud Present Regionally restricted 5B Expression is at the proximal region.

Specimen 5C: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: digit mesenchyme Present Regionally restricted 5C Expression is in the area of tarsal/carpal condensations.

Specimen 5D: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: forelimb phalanx pre-cartilage condensation Present 5D
TS21: metacarpus pre-cartilage condensation Present 5D
TS21: forelimb interdigital region Absent 5D

Specimen 5E: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: limb mesenchyme Present Regionally restricted 5E Expression is in mesenchymal cells surrounding the differentiating cartilage.
TS21: limb digit Present Regionally restricted 5E Expression is in bones at the distal end of the digits.

Specimen not shown E9.5: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: future forebrain Strong
TS15: future hindbrain Absent

Specimen not shown E10.5: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted Expression is at the tip of the distal region.

Specimen not shown Sey-/+: embryonic day 10.5; Pax6Sey/Pax6+ (more )
Structure Level Pattern Image Note
TS17: diencephalon Strong

Specimen not shown Sey-/-: embryonic day 10.5; Pax6Sey/Pax6Sey (more )
Structure Level Pattern Image Note
TS17: diencephalon Absent


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/28/2024
MGI 6.13
The Jackson Laboratory