Probe:	sFRP-1
Probe preparation:	labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	5A	Expression first detected in anterior ectoderm just ahead of the forward-migrating head process.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	5B	Expression first detected in anterior ectoderm just ahead of the forward-migrating head process.

Structure	Level	Pattern	Image	Note
TS13: optic pit neural ectoderm	Present	Regionally restricted	5C	Expression in neural ectoderm of the posterior region of the optic eminences.
TS13: future hindbrain neural ectoderm	Present	Regionally restricted	5C	Expression in ventral hindbrain.
TS13: rhombomere	Present	Regionally restricted	5C	Expression in rhombomeres in and alternated pattern, with lower levels of expression in rhombomeres 3 and 5

Structure	Level	Pattern	Image	Note
TS14: eye	Present	Regionally restricted	5D	Expression persists in the posterior region of the eye.
TS14: future hindbrain	Present	Regionally restricted	5D	Expression persists in the ventral hindbrain.

Structure	Level	Pattern	Image	Note
TS15: eye	Present	Regionally restricted	5E	Expression persists in the posterior region of the eye.
TS15: future hindbrain	Present	Regionally restricted	5E	Anterior limit of expression in the ventral hindbrain corresponds to the junction of midbrain and hindbrain.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted		Authors state expression is similar to that seen in 9.5 dpc embryos.