Gene Expression Data
Assay Details
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Assay
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Reference:
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J:66436
Moorman AF, et al., Sensitive nonradioactive detection of mRNA in tissue sections. Novel application of the whole-mount in situ hybridization protocol. J Histochem Cytochem. 2001 Jan;49(1):1-8
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5688597
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Gene symbol:
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Myl2
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Gene name:
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myosin, light polypeptide 2, regulatory, cardiac, slow
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Probe:
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MLC-2v probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2B:
embryonic day 13.5
(more  )
Note: Section hybridized at 58 degrees.
Specimen
2B:
(close  )
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Genetic Background:
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FVB
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Section hybridized at 58 degrees.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: heart |
Present |
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2B
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Specimen
2B':
embryonic day 13.5
(more )
Note: Section hybridized at 70 degrees.
Specimen
2B':
(close )
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Genetic Background:
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FVB
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Section hybridized at 70 degrees.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: heart |
Present |
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2B'
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The signal is more specific at the higher temperature.
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Analysis Tools
