Probe:	Glis1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS12: extraembryonic mesenchyme	Present		5A

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present		5B

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	5C	Expression is in facial mesenchyme and as a stripe below the telencephalic vesicles.
TS17: branchial arch	Present		5C
TS17: genital swelling	Present	Regionally restricted	5C	Expression is in the proximal genital tubercle.
TS17: tail mesenchyme	Present	Regionally restricted	5C	Expression is in the ventral mesenchyme.
TS17: dermomyotome	Present		5C	Expression is in the hypaxial myotome in the more caudal regions.

Structure	Level	Pattern	Image	Note
TS17: genital swelling	Present	Regionally restricted	5D	Expression is in the proximal genital tubercle.
TS17: somite	Present		5D
TS17: tail mesenchyme	Present	Regionally restricted	5D	Expression is in the ventral mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: dermomyotome	Present		5E

Structure	Level	Pattern	Image	Note
TS17: dermomyotome	Present	Regionally restricted	5F	Expression is in the hypaxial myotome in the more caudal regions.

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Present	Regionally restricted	5G	Expression is in a domain below the telencepahlic vesicles that extends posteriorly and is continuous with expression in the fused nasal and maxillary processes
TS19: maxillary process	Present		5G
TS19: nasal process	Present		5G
TS19: nasal process mesenchyme	Absent		5G	Absent in the nasal capsule.

Structure	Level	Pattern	Image	Note
TS22: nose	Present	Regionally restricted	5H	Expression is in the frontal nasal region.

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Present		5I
TS22: vibrissa follicle	Present		5I
TS22: nose	Present	Regionally restricted	5I	Expression is in the frontal nasal region.

Structure	Level	Pattern	Image	Note
TS15: hindlimb ridge mesenchyme	Present	Regionally restricted	5J	Expression is in the anterior-proximal mesenchyme.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present	Regionally restricted	5K	Expression is in the anterior-proximal mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	5L	Expression is in the anterior-proximal mesenchyme in 2/3rd of the limb and in a posterior region.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present		5M	The anterior expression domain extends along the entire limb and is continuous with expression in the mesenchyme underlying the apical ectodermal ridge.
TS19: handplate mesenchyme	Present	Regionally restricted	5M	Expression is in two domains at the anterior and posterior base and in mesenchyme underlying the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS20: hand mesenchyme	Present	Regionally restricted	5N	Expression is in two domains at the anterior and posterior base.

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	5O	Expression is in two domains at the anterior and posterior base.

Structure	Level	Pattern	Image	Note
TS22: hand skin	Present	Regionally restricted	5P	Expression is on the ventral limb surface.
TS22: hair follicle	Present		5P
TS22: forelimb interphalangeal joint	Present	Regionally restricted	5P	Expression is in the joint interzone.

Structure	Level	Pattern	Image	Note
TS22: hand skin	Present	Regionally restricted	5Q	Expression is on the ventral limb surface.
TS22: forelimb interphalangeal joint	Present	Regionally restricted	5Q	Expression is in the joint interzone.

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS17: branchial arch mesenchyme	Present	Regionally restricted		Expression is distal and is stronger in the posterior arch.

Structure	Level	Pattern	Image	Note
TS21: vibrissa follicle	Present