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Gene Expression Data
Assay Details
Assay
Reference: J:153562 Acampora D, et al., Selective inactivation of Otx2 mRNA isoforms reveals isoform-specific requirement for visceral endoderm anteriorization and head morphogenesis and highlights cell diversity in the visceral endoderm. Mech Dev. 2009 Oct;126(10):882-97
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5688184
Gene symbol: Otx2
Gene name: orthodenticle homeobox 2
Antibody: anti-OTX2
Assay notes: Two antibodies were described MGI:1309322 and MGI:5662473; the one used in this assay was not specified.
Results
Specimen 3I: embryonic day 6.5 (more )
Note: Double labeled: red - Otx2; green - Lhx1 (assay MGI:5688189).
Structure Level Pattern Image Note
TS8: anterior visceral endoderm Present 3I
TS8: epiblast Present 3I
Specimen 3I': embryonic day 6.5; Otx2tm9Asim/Otx2tm11Asim (more )
Note: Double labeled: red - Otx2; green - Lhx1 (assay MGI:5688189).
Structure Level Pattern Image Note
TS8: epiblast Weak 3I'
TS8: anterior visceral endoderm Weak Regionally restricted 3I' Some cells showed a reduction of expression in the mutant. However, the visceral endoderm marker Lhx1 was expressed normally.
Specimen 6F: embryonic day 6.5 (more )
Note: Double labeled: red - Otx2; green - Lhx1 (assay MGI:5688189).
Structure Level Pattern Image Note
TS8: primitive endoderm Present 6F
Specimen 6J: embryonic day 6.5; Otx2tm10Asim/Otx2tm11Asim (more )
Note: Double labeled: red - Otx2; green - Lhx1 (assay MGI:5688189).
Structure Level Pattern Image Note
TS8: primitive endoderm Present Single cells 6J Only a few visceral endoderm cells retained immunostaining in the mutant compared to wild type.
Specimen 9C: embryonic day 6.5; Otx2tm1Pas/Otx2+ (more )
Note: Double labeled: red - Otx2 protein; green - Otx2 reporter (assay MGI:5688750).
Structure Level Pattern Image Note
TS8: primitive endoderm Present 9C Most, but not all, immunopositive visceral endoderm cells were also beta-galactosidase-positive.
Specimen 9F: embryonic day 6.5; Otx2tm1Pas/Otx2tm9Asim (more )
Note: Double labeled: red - Otx2 protein; green - Otx2 reporter (assay MGI:5688750).
Structure Level Pattern Image Note
TS8: primitive endoderm Present 9F The majority of immunopositive cells were beta-galactosidase-positive.
Specimen 9I: embryonic day 6.5; Otx2tm1Pas/Otx2tm10Asim (more )
Note: Double labeled: red - Otx2 protein; green - Otx2 reporter (assay MGI:5688750).
Structure Level Pattern Image Note
TS8: primitive endoderm Present Single cells 9I A few cells were immunopositive, some were beta-galactosidase-positive and some were negative.
Specimen 9L: embryonic day 6.5; Otx2tm1Pas/Otx2tm10Asim (more )
Note: Double labeled: red - Otx2 protein; green - Otx2 reporter (assay MGI:5688750).
Structure Level Pattern Image Note
TS8: primitive endoderm Present Single cells 9L A few cells were immunopositive, some were beta-galactosidase-positive and some were negative.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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