Probe:	Fgf8 cDNA2
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS8: primitive endoderm	Present		1a

Structure	Level	Pattern	Image	Note
TS9: primitive endoderm	Present		1b
TS9: embryo mesoderm	Present	Regionally restricted	1b	Expression is in the primitive streak region.
TS9: embryo ectoderm	Present	Regionally restricted	1b	Expression is in the primitive streak region, absent in anterior ectoderm.

Structure	Level	Pattern	Image	Note
TS9: primitive endoderm	Present		1c
TS9: embryo mesoderm	Present	Regionally restricted	1c	Expression is in the primitive streak region.
TS9: embryo ectoderm	Present	Regionally restricted	1c	Expression is in the primitive streak region, absent in anterior ectoderm.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present	Regionally restricted	1d	Expression is absent in the most anterior part.
TS12: neural ectoderm	Present	Regionally restricted	1d	Expression is in the a region of prechordal neuroepithelium and the prechordal floorplate.
TS12: cardiogenic plate	Present		1d
TS12: embryo endoderm	Present	Regionally restricted	1d	Expression is in the endoderm around the intraembryonic coleom.
TS12: head mesenchyme	Present	Regionally restricted	1d	Expression is in a restricted area of the cranial apraxial mesoderm.
TS12: surface ectoderm	Present	Regionally restricted	1d	Expression is in the head surface ectoderm in a restricted area.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present	Regionally restricted	1e	Expression is absent in the most anterior part.
TS12: branchial arch	Present		1e
TS12: future midbrain	Present		1e

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present	Regionally restricted	1f	Expression is absent in the most anterior part.
TS13: 1st branchial pouch endoderm	Present		1f
TS13: 2nd branchial pouch endoderm	Present		1f
TS13: branchial arch ectoderm	Present		1f
TS13: midbrain-hindbrain junction	Present		1f
TS13: future prosencephalon	Present	Regionally restricted	1f	Expression is at the rostral tip around the neuropore.
TS13: surface ectoderm	Present	Regionally restricted	1f	Expression is in the facial ectoderm adjacent to, and continuous with, that in the rostral forebrain.

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Present		1g
TS15: tail	Present		1g
TS15: somite	Present	Regionally restricted	1g	Expression is as two dorso-ventrally oriented stripes within each somite.
TS15: telencephalon	Present	Regionally restricted	1g	Expression is at the rostral tip around the neuropore.
TS15: forelimb bud	Present	Regionally restricted	1g	Expression is distal.
TS15: hindlimb ridge	Present	Regionally restricted	1g	Expression is distal.

Structure	Level	Pattern	Image	Note
TS16: midbrain-hindbrain junction	Present		1h
TS16: tail	Present		1h
TS16: somite	Present	Regionally restricted	1h	Expression is as two dorso-ventrally oriented stripes within each somite.
TS16: future forebrain	Present	Regionally restricted	1h	Expression is in the dorsal region of the midbrain-forebrain boundary.
TS16: future midbrain	Present	Regionally restricted	1h	Expression is in the dorsal region of the midbrain-forebrain boundary.
TS16: forelimb bud	Present	Regionally restricted	1h	Expression is distal.
TS16: hindlimb bud	Present	Regionally restricted	1h	Expression is distal.

Structure	Level	Pattern	Image	Note
TS15: tail mesenchyme	Present		1i
TS15: tail neural plate	Present		1i
TS15: tail surface ectoderm	Present		1i

Structure	Level	Pattern	Image	Note
TS15: tail mesenchyme	Present		1j
TS15: tail neural plate	Present		1j
TS15: tail surface ectoderm	Present		1j

Structure	Level	Pattern	Image	Note
TS15: tail mesenchyme	Present		1k
TS15: tail neural plate	Present		1k
TS15: tail surface ectoderm	Present		1k

Structure	Level	Pattern	Image	Note
TS14: branchial arch	Present		1l
TS14: cardiac muscle tissue	Weak		1l

Structure	Level	Pattern	Image	Note
TS15: myotome	Present	Regionally restricted	1m	Expression is in the anterior and posterior margins of the myotome.

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Present	Regionally restricted	1n	Expression is between the ventricular and pial surfaces of the neuroepithelium and is absent in the floor plate and roof plate.

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Present	Regionally restricted	1o	Expression is between the ventricular and pial surfaces of the neuroepithelium and is absent in the floor plate and roof plate.

Structure	Level	Pattern	Image	Note
TS16: future forebrain	Present	Regionally restricted	1p	Expression is at the neuropore region and in the dorsal region at the junction of the forebrain/midbrain.
TS16: future midbrain	Present	Regionally restricted	1p	Expression is in the dorsal region at the junction of the forebrain/midbrain.
TS16: telencephalon	Present	Regionally restricted	1p	Expression is at the rostral tip around the neuropore.
TS16: surface ectoderm	Present	Regionally restricted	1p	Expression is in the facial ectoderm adjacent to, and continuous with, that in the rostral forebrain.

Structure	Level	Pattern	Image	Note
TS22: midbrain ventricular layer	Present	Regionally restricted	1q	Expression is in the ventro-posterior midbrain.
TS22: forebrain	Absent		1q
TS22: midbrain-hindbrain junction	Absent		1q

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component ectoderm	Present		1r
TS15: 1st branchial arch maxillary component ectoderm	Present		1r

Structure	Level	Pattern	Image	Note
TS15: 2nd branchial arch ectoderm	Present		1s
TS15: 2nd branchial pouch endoderm	Present		1s
TS15: 3rd branchial arch ectoderm	Present		1s
TS15: 3rd branchial pouch endoderm	Present		1s

Structure	Level	Pattern	Image	Note
TS15: 2nd branchial arch ectoderm	Present		1t
TS15: 2nd branchial pouch endoderm	Present		1t

Structure	Level	Pattern	Image	Note
TS18: 1st branchial arch mandibular component	Present		1u
TS18: apical ectodermal ridge	Present		1u
TS18: latero-nasal process	Present		1u
TS18: nasal pit	Present		1u
TS18: forebrain	Present	Regionally restricted	1u	Expression is in the anterior forebrain.
TS18: limb	Present	Regionally restricted	1u	Expression is in the condensing cartilage at the base of the limb.
TS18: telencephalon	Present	Regionally restricted	1u	Expression is in the olfactory bulb primordium.

Structure	Level	Pattern	Image	Note
TS22: maturing glomerular tuft	Present		1v

Structure	Level	Pattern	Image	Note
TS15: limb ectoderm	Present	Regionally restricted	3a	Expression is in the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS14: forelimb bud ectoderm	Present	Regionally restricted	3b	Expression is in the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS14: forelimb bud ectoderm	Present	Regionally restricted	3c	Expression is in the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS14: somite	Present	Regionally restricted		Expression is in anterior somites.

Structure	Level	Pattern	Image	Note
TS17: infundibular recess of 3rd ventricle	Present
TS17: tail somite	Present
TS17: trunk somite	Absent

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present