Gene Expression Data
Assay Details
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Assay
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Reference:
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J:65743
Harrison SM, et al., Sp5, a new member of the Sp1 family, is dynamically expressed during development and genetically interacts with brachyury. Dev Biol. 2000 Nov 15;227(2):358-72
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|
Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5661901
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Gene symbol:
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Sp5
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Gene name:
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trans-acting transcription factor 5
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Probe:
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Sp5 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
3A-1:
embryonic day 5.5
(more  )
Specimen
3A-1:
(close  )
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Genetic Background:
|
involves: C57BL/6 * DBA
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|
Age:
|
embryonic day 5.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3A-2:
embryonic day 6.0
(more )
Specimen
3A-2:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
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|
Age:
|
embryonic day 6.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3A-3:
embryonic day 6.5
(more )
Note: Early streak stage.
Specimen
3A-3:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
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|
Age:
|
embryonic day 6.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Early streak stage.
|
Specimen
3A-4:
embryonic day 6.5
(more )
Note: Early streak stage.
Specimen
3A-4:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 6.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Early streak stage.
|
Specimen
3B:
embryonic day 6.5
(more )
Note: Early streak stage.
Specimen
3B:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 6.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Early streak stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS9: primitive streak |
Present |
|
3B
|
Expression extended distally with the primitive streak and laterally with the formation of the nascent mesoderm.
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Specimen
3C:
embryonic day 6.5
(more )
Note: Early streak stage.
Specimen
3C:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 6.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Early streak stage.
|
Specimen
3D:
embryonic day 7.0
(more )
Note: Late streak stage.
Specimen
3D:
(close )
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Genetic Background:
|
involves: C57BL/6 * DBA
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|
Age:
|
embryonic day 7.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Late streak stage.
|
Specimen
4A:
embryonic day 7.5
(more )
Note: Neural plate stage.
Specimen
4A:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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|
Sex:
|
Not Specified
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|
Type:
|
whole mount
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|
Fixation:
|
4% Paraformaldehyde
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|
Note:
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Neural plate stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: primitive streak |
Present |
|
4A
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| TS11: embryo mesoderm |
Present |
Regionally restricted |
4A
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Expressed in paraxial mesoderm, which lies beneath the cranial neural folds in the region of the prospective midbrain.
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Specimen
4B:
embryonic day 8.0
(more )
Note: 2-somite stage.
Specimen
4B:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
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2-somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: primitive streak |
Present |
|
4B
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|
| TS12: future midbrain |
Present |
Regionally restricted |
4B
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Expressed in a broad dorsoventral domain at the level of the prospective midbrain comprising not only neurectoderm and paraxial mesoderm but also definitive gut endoderm.
|
Specimen
4C:
embryonic day 8.0
(more )
Note: 6-somite stage.
Specimen
4C:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
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|
Note:
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6-somite stage.
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Specimen
4D:
embryonic day 8.0
(more )
Note: 6-somite stage.
Specimen
4D:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
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|
Note:
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6-somite stage.
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Specimen
4E:
embryonic day 9.5
(more )
Note: 22-somite stage.
Specimen
4E:
(close )
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Genetic Background:
|
involves: C57BL/6 * DBA
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|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
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|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
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|
Note:
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22-somite stage.
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Specimen
4F:
embryonic day 11.0
(more )
Note: 40-somite stage.
Specimen
4F:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
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40-somite stage.
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Specimen
4G:
embryonic day 11.0
(more )
Note: 40-somite stage.
Specimen
4G:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
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|
Note:
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40-somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: telencephalon |
Strong |
Regionally restricted |
4G
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Expressed in a narrow stripe at the posterior aspect of each telencephalic vesicle.
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| TS18: diencephalon |
Strong |
Regionally restricted |
4G
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Expressed in a broad dorsal domain.
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Specimen
4H:
embryonic day 11.0
(more )
Note: 40-somite stage.
Specimen
4H:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
40-somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: midbrain |
Strong |
Regionally restricted |
4H
|
Expressed in two disctete dorsal stripes lateral to the roof plate.
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| TS18: cerebellum primordium |
Strong |
Regionally restricted |
4H
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Expressed as a lateral stripe.
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Specimen
4I:
embryonic day 8.5
(more )
Note: 12-somite stage.
Specimen
4I:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
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|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
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Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
12-somite stage.
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Specimen
4J:
embryonic day 8.5
(more )
Note: 12-somite stage.
Specimen
4J:
(close )
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Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
12-somite stage.
|
Specimen
4K:
embryonic day 8.5
(more )
Note: 12-somite stage.
Specimen
4K:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
12-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural tube |
Present |
Regionally restricted |
4K
|
Dorsal expression.
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| TS13: somite |
Present |
Regionally restricted |
4K
|
Dorsal expression.
|
Specimen
4L:
embryonic day 8.5
(more )
Note: 12-somite stage.
Specimen
4L:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
12-somite stage.
|
Specimen
4M:
embryonic day 8.5
(more )
Note: 12-somite stage.
Specimen
4M:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
12-somite stage.
|
Specimen
4N:
embryonic day 8.5
(more )
Note: 12-somite stage.
Specimen
4N:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
12-somite stage.
|
Specimen
5A:
embryonic day 11.0
(more )
Note: 40-somite stage.
Specimen
5A:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
40-somite stage.
|
Specimen
5B:
embryonic day 11.0
(more )
Note: 40-somite stage.
Specimen
5B:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
40-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: dermomyotome |
Present |
Regionally restricted |
5B
|
Expressed in the dorsomedial and ventrolateral lips.
|
Specimen
5E:
embryonic day 9.5
(more )
Note: 22-somite stage.
Specimen
5E:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
22-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: dermomyotome |
Present |
Regionally restricted |
5E
|
Expressed in the ventrolateral dermamyotome adjacent to the limb bud.
|
Specimen
5F:
embryonic day 9.5
(more )
Note: 26-somite stage.
Specimen
5F:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
26-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: somite |
Present |
Regionally restricted |
5F
|
Expressed in dispersed and presumably migrating cells adjacent to the forelimb bud.
|
Specimen
5G:
embryonic day 11.0
(more )
Note: 40-somite stage.
Specimen
5G:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
40-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: dermomyotome |
Present |
Regionally restricted |
5G
|
Expressed in the dorsomedial and ventrolateral dermamyotome.
|
| TS18: limb |
Present |
Regionally restricted |
5G
|
Proximally expressed within the limb bud.
|
Specimen
5I:
embryonic day 11.0
(more )
Note: 40-somite stage.
Specimen
5I:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
40-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: limb |
Present |
Regionally restricted |
5I
|
Expressed dorsally and ventrally within the limb. Transcripts are localised more superficially than Pax3.
|
Specimen
6A:
embryonic day 9.5-10.0
(more )
Note: 28-30-somite stage.
Specimen
6A:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 9.5-10.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
28-30-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Present |
Regionally restricted |
6A
|
Proximally expressed within the limb bud.
|
Specimen
6C:
embryonic day 9.5-10.0; Pax3Sp-2H/ Pax3Sp-2H
(more )
Note: 28-30-somite stage.
Specimen
6C:
(close )
|
Genetic Background:
|
involves: 101 * C3H/He
|
|
Mutant Allele(s):
|
Pax3Sp-2H/Pax3Sp-2H
|
|
Age:
|
embryonic day 9.5-10.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
28-30-somite stage.
|
Specimen
6D:
embryonic day 9.5-10.0
(more )
Note: 28-30-somite stage.
Specimen
6D:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 9.5-10.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
28-30-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Present |
Regionally restricted |
6D
|
Expressed as discrete dorsal and ventral patches within the limb bud.
|
Specimen
6F:
embryonic day 9.5-10.0; Pax3Sp-2H/ Pax3Sp-2H
(more )
Note: 28-30-somite stage.
Specimen
6F:
(close )
|
Genetic Background:
|
involves: 101 * C3H/He
|
|
Mutant Allele(s):
|
Pax3Sp-2H/Pax3Sp-2H
|
|
Age:
|
embryonic day 9.5-10.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
28-30-somite stage.
|
Specimen
not shown:
embryonic day 13.5
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: tail |
Present |
|
|
|
Specimen
not shown:
embryonic day 11.0
(more )
Note: 40-somite stage.
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
40-somite stage.
|
Specimen
not shown:
embryonic day 7.0
(more )
Note: Late streak stage.
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 7.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Late streak stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo mesoderm |
Present |
Regionally restricted |
|
Expressed as two bilaterally symmetrical spurs of paraxial mesoderm.
|
Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: dermomyotome |
Present |
Regionally restricted |
|
Expressed in the dorsomedial and ventrolateral lips.
|
Specimen
not shown:
embryonic day 14.5
(more )
Note: 60-somite stage.
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
60-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hand |
Present |
Regionally restricted |
|
Expressed in a small population of cells in the palmar surface.
|
|
Analysis Tools