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Gene Expression Data
Assay Details
Assay
Reference: J:85486 Xiao J, et al., Timeless in lung morphogenesis. Dev Dyn. 2003 Sep;228(1):82-94
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5661692
Gene symbol: Timeless
Gene name: timeless circadian clock 1
Antibody: anti-Timeless
Detection system: Secondary antibody coupled to horseradish peroxidase
Results
Specimen 1B: embryonic day 10.0 (more )
Structure Level Pattern Image Note
TS16: future forebrain Strong 1B
TS16: future hindbrain roof plate Strong 1B
TS16: neural tube Strong 1B
TS16: olfactory epithelium Strong 1B
TS16: optic stalk Strong 1B
TS16: tail future spinal cord Strong 1B
TS16: future brain Present 1B
TS16: heart Present 1B
TS16: liver Present 1B
TS16: lung Present 1B
TS16: urogenital ridge Present 1B
TS16: foregut Present Regionally restricted 1B Expression is in the stomach.
TS16: future midbrain Strong 1B inset , 1B

Specimen 1C: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: metanephros Present 1C

Specimen 1D: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: lung Present 1D

Specimen 2A: embryonic day 13.0 (more )
Note: (B) is a higher magnification of (A).
Structure Level Pattern Image Note
TS21: lung epithelium Present 2B , 2A Staining is predominantly nuclear.
TS21: lung mesenchyme Present 2B , 2A Staining is predominantly nuclear.

Specimen 3A: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: lung epithelium Present 3A Staining is nuclear.
TS18: lung mesenchyme Present 3A Staining is nuclear.

Specimen 3B: embryonic day 15.0 (more )
Structure Level Pattern Image Note
TS23: lung epithelium Present 3B Staining is nuclear.
TS23: lung mesenchyme Present 3B Staining is nuclear.

Specimen 3C: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: bronchiole epithelium Strong Regionally restricted 3C Expression is in the epithelium of distal airways and saccular epithelium.
TS26: lung epithelium Strong Regionally restricted 3C Expression is in the epithelium of distal airways and saccular epithelium.
TS26: lung mesenchyme Weak Non-Uniform 3C
TS26: lung vascular element Present Regionally restricted 3C Expression is in endothelial cells.

Specimen not shown: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: bronchus epithelium Strong
TS26: trachea epithelium Strong

Specimen 3D: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: lung epithelium Strong Regionally restricted 3D Expression is in the epithelium of distal airways and saccular epithelium.
TS27: lung Weak Regionally restricted 3D Non-uniform distribution in the mesenchyme.

Specimen 3E: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: lung Trace Regionally restricted 3E Expression is nearly absent in the mesenchyme.
TS28: lung epithelium Present Regionally restricted 3E Expression is in a subset of cells surrounding the saccular or prealveolar structures.

Specimen 3F: postnatal adult (more )
Structure Level Pattern Image Note
TS28: lung epithelium Present Regionally restricted 3F Expression is in a subset of cells surrounding the saccular or prealveolar structures.

Specimen 5B: embryonic day 18.0 (more )
Note: Double labeled: brown - Timeless; blue - Scgb1a1 (assay MGI:5661696).
Structure Level Pattern Image Note
TS26: bronchiole epithelium Present 5B

Specimen 5D: embryonic day 18.0 (more )
Note: Double labeled: brown - Timeless; blue - Acta2 (assay MGI:5661697).
Structure Level Pattern Image Note
TS26: bronchiole epithelium Present 5D
TS26: lung vascular element Present 5D

Specimen 10C,D: postnatal day 1 (more )
Note: (D) is the same section as (C) counter stained with Hematoxylin and Eosin.
Structure Level Pattern Image Note
TS27: lung epithelium Present 10D , 10C


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/16/2024
MGI 6.23
The Jackson Laboratory