Probe:	Irx5 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present			Expression is present in the neural epithelium of the head fold.
TS12: splanchnopleure	Present			Expression is present in the lateral plate mesoderm lining the coelom.

Structure	Level	Pattern	Image	Note
TS20: endocardial lining	Weak		1B Irx5
TS20: head mesenchyme	Present		1B Irx5
TS20: heart	Present		1B Irx5
TS20: facial ganglion	Present		1B Irx5
TS20: vestibulo-cochlear ganglion	Present		1B Irx5
TS20: metencephalon	Present		1B Irx5
TS20: spinal cord	Present		1B Irx5	Expression is present in the neural tube.
TS20: otic capsule	Present		1B Irx5
TS20: skin	Present		1B Irx5

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	2E	Expression is present dorsolaterally.
TS19: metencephalon	Present	Regionally restricted	2E	Expression is present ventrolaterally.
TS19: metencephalon basal plate	Strong	Regionally restricted	2E	Expression is found in the sensory region and lateral region of the motor part of the pons.
TS19: cerebellum primordium	Weak		2E

Structure	Level	Pattern	Image	Note
TS19: neural tube ventricular layer	Present		2K
TS19: neural tube alar columns	Present		2K	Expression is in the mantle layer.
TS19: neural tube basal columns	Present		2K	Expression is in the mantle layer.
TS19: neural tube lateral wall	Present		2K	Expression is present in motor neurons.
TS19: neural tube floor plate	Absent		2K
TS19: neural tube roof plate	Absent		2K
TS19: notochord	Present		2K

Structure	Level	Pattern	Image	Note
TS19: inner ear	Present		2Q	Expression is present in the otic vesicle.
TS19: facial ganglion	Present	Regionally restricted	2Q	Expression is present in the ganglia medial to the cranial cardinal vein.

Structure	Level	Pattern	Image	Note
TS21: dermis	Weak		3A
TS21: epidermis	Weak		3A
TS21: mammary gland primordium	Present		3A

Structure	Level	Pattern	Image	Note
TS21: lower jaw molar	Present		3L
TS21: upper jaw molar	Present		3L
TS21: oral epithelium	Present		3L	Expression extends lateral from the molar tooth primordia.

Structure	Level	Pattern	Image	Note
TS15: limb mesenchyme	Weak	Regionally restricted		Expression is present in the proximal region over the antero-posterior axis, highest at the dorsal side.

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Weak	Regionally restricted		Expression is present proximally.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Weak	Regionally restricted	4B E11.5	Expression is present in the proximal limb.
TS19: hindlimb bud mesenchyme	Weak	Regionally restricted	4B E11.5	Expression is present in the proximal limb.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present	Regionally restricted	4B E12.5	Expression is present in the proximal limb.
TS20: hindlimb mesenchyme	Present	Regionally restricted	4B E12.5	Expression is present in the proximal limb.

Structure	Level	Pattern	Image	Note
TS21: forelimb interdigital region mesenchyme	Present		4B E13.5
TS21: hindlimb interdigital region mesenchyme	Present		4B E13.5

Structure	Level	Pattern	Image	Note
TS22: forelimb interdigital region mesenchyme	Present		4B E14.5
TS22: hindlimb interdigital region mesenchyme	Present		4B E14.5

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	4G	Expression is present in the proximal region.

Structure	Level	Pattern	Image	Note
TS21: forelimb interdigital region mesenchyme	Present		4I

Structure	Level	Pattern	Image	Note
TS19: trachea epithelium	Present		5E	Expression in the laryncho-tracheal groove endoderm.
TS19: trachea mesenchyme	Absent		5E	No expression in the laryncho-tracheal groove mesenchyme.
TS19: foregut epithelium	Present		5E	Expression in foregut endoderm.
TS19: atrium endocardial lining	Present		5E

Structure	Level	Pattern	Image	Note
TS14: endocardial tube	Present			Expression is present in the endocardium.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Regionally restricted		Expression is medial, adjacent to the hindbrain.

Structure	Level	Pattern	Image	Note
TS17: lung epithelium	Present
TS17: lung mesenchyme	Absent

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium	Absent
TS19: gonad	Weak
TS19: acoustic ganglion	Present			Expression is present in the complex.
TS19: facial ganglion	Present			Expression is present in the complex.

Structure	Level	Pattern	Image	Note
TS21: nasal capsule	Present			Expression is present in the cartilage.
TS21: stomach	Present			Expression is present in the endoderm.
TS21: gonad	Weak
TS21: lung epithelium	Present
TS21: lung mesenchyme	Absent
TS21: neural retinal epithelium	Present	Regionally restricted		Expression is present in the marginal zone.
TS21: thalamus	Present
TS21: vibrissa follicle	Weak			Expression is in the germinal matrix.
TS21: endocardial tissue	Present	Regionally restricted		There is expression in the atrial and ventricular endodcardium, but not in the endocardium of the cushions.
TS21: atrium endocardial lining	Present
TS21: ventricle endocardial lining	Present